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Search results: CD19 Protein

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Product group Antibodies
LifeSpan BioSciences
ApplicationsFlow Cytometry, ImmunoFluorescence
ReactivityHuman
TargetCD19
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
ApplicationsFlow Cytometry, ImmunoFluorescence
ReactivityHuman
TargetCD19
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, ImmunoHistoChemistry, Neutralisation/Blocking
ReactivityHuman
TargetCD19
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, ImmunoHistoChemistry, Neutralisation/Blocking
ReactivityHuman
TargetCD19
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
ApplicationsFlow Cytometry, ImmunoFluorescence
ReactivityHuman
TargetCD19
  • SizePrice
Product group Chemicals
MedChem Express
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Product group Chemicals
TargetMol Chemicals
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Product group Chemicals
TargetMol Chemicals
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Figure 1. Western blot analysis of CD19 using anti-CD19 antibody (A00154-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse spleen tissue lysates, Lane 2: mouse thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD19 antigen affinity purified polyclonal antibody (Catalog # A00154-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD19 at approximately 85KD. The expected band size for CD19 is at 61KD.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse
TargetCD19
  • SizePrice
Figure 1. Western blot analysis of CD19 using anti-CD19 antibody (A00154-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates, Lane 2: rat thymus tissue lysates, Lane 3: mouse spleen tissue lysates, Lane 4: mouse thymus tissue lysates, Lane 5: human K562 whole cell lysates, Lane 6: human 22RV1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD19 antigen affinity purified polyclonal antibody (Catalog # A00154-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD19 at approximately 85KD. The expected band size for CD19 is at 61KD.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetCd19
  • SizePrice
Figure 1. Western blot analysis of CD19 using anti-CD19 antibody (PB9800). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human Ramos whole cell lysates, Lane 3: human Daudi whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD19 antigen affinity purified polyclonal antibody (Catalog # PB9800) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD19 at approximately 95 kDa. The expected band size for CD19 is at 61 kDa.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman
TargetCD19
  • SizePrice

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