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Search results: CDC25B

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Figure 1. Western blot analysis of Cdc25B using anti-Cdc25B antibody (PA1546). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: Rat Brain Tissue Lysate, Lane 2: Rat Kidney Tissue Lysate, Lane 3: Rat Lung Tissue Lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cdc25B antigen affinity purified polyclonal antibody (Catalog # PA1546) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cdc25B at approximately 65 kDa. The expected band size for Cdc25B is at 65 kDa.
Product group Antibodies
Boster Bio
Anti-M-phase inducer phosphatase 2 Cdc25B Antibody Picoband(r)PA1546-HRP
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityCanine, Equine, Human, Monkey, Rabbit, Rat
TargetCDC25B
  • SizePrice
Figure 1. Western blot analysis of Cdc25B using anti-Cdc25B antibody (PA1546). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: Rat Brain Tissue Lysate, Lane 2: Rat Kidney Tissue Lysate, Lane 3: Rat Lung Tissue Lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cdc25B antigen affinity purified polyclonal antibody (Catalog # PA1546) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cdc25B at approximately 65 kDa. The expected band size for Cdc25B is at 65 kDa.
Product group Antibodies
Boster Bio
Anti-M-phase inducer phosphatase 2 Cdc25B Antibody Picoband(r)PA1546-IFLUOR647
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityCanine, Equine, Human, Monkey, Rabbit, Rat
TargetCDC25B
  • SizePrice
Figure 1. Western blot analysis of Cdc25B using anti-Cdc25B antibody (PA1546). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: Rat Brain Tissue Lysate, Lane 2: Rat Kidney Tissue Lysate, Lane 3: Rat Lung Tissue Lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cdc25B antigen affinity purified polyclonal antibody (Catalog # PA1546) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cdc25B at approximately 65 kDa. The expected band size for Cdc25B is at 65 kDa.
Product group Antibodies
Boster Bio
Anti-M-phase inducer phosphatase 2 Cdc25B Antibody Picoband(r)PA1546-PE
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityCanine, Equine, Human, Monkey, Rabbit, Rat
TargetCDC25B
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
CDC25B Antibody (aa331-360, APC)LS-C254249
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetCDC25B
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
CDC25B Antibody (aa331-360, Biotin)LS-C254250
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetCDC25B
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
CDC25B Antibody (aa331-360, FITC)LS-C254251
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetCDC25B
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
CDC25B Antibody (aa331-360, PE)LS-C254253
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetCDC25B
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
CDC25B Antibody (aa165-194, APC)LS-C254255
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetCDC25B
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
CDC25B Antibody (aa165-194, Biotin)LS-C254256
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetCDC25B
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
CDC25B Antibody (aa165-194, FITC)LS-C254257
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetCDC25B
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
CDC25B Antibody (aa165-194, PE)LS-C254259
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetCDC25B
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
CDC25B Antibody (aa377-566, Biotin)LS-C301213
ApplicationsWestern Blot
ReactivityHuman
TargetCDC25B
  • SizePrice
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