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Search results: CDC25B

337 to 348 of 1.161 products
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Product group Antibodies
Biorbyt
ReactivityHuman, Mouse
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Product group Proteins / Signaling Molecules
Biorbyt
Protein IDP30305
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Product group Proteins / Signaling Molecules
Biorbyt
Protein IDP30305
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Product group Antibodies
Biorbyt
ApplicationsWestern Blot, ELISA
ReactivityHuman
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Product group DNA / RNA / Vectors
OriGene
CategoryPlasmid / vector
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Product group DNA / RNA / Vectors
OriGene
CategoryPlasmid / vector
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Product group DNA / RNA / Vectors
OriGene
CategoryPlasmid / vector
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Product group DNA / RNA / Vectors
OriGene
CategoryPlasmid / vector
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Product group DNA / RNA / Vectors
OriGene
CategoryPlasmid / vector
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Product group DNA / RNA / Vectors
OriGene
CategoryPlasmid / vector
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Product group DNA / RNA / Vectors
OriGene
CategoryPlasmid / vector
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Figure 1. Western blot analysis of Cdc25B using anti-Cdc25B antibody (PA1546). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: Rat Brain Tissue Lysate, Lane 2: Rat Kidney Tissue Lysate, Lane 3: Rat Lung Tissue Lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cdc25B antigen affinity purified polyclonal antibody (Catalog # PA1546) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cdc25B at approximately 65 kDa. The expected band size for Cdc25B is at 65 kDa.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityCanine, Equine, Human, Monkey, Rabbit, Rat
TargetCDC25B
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