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Search results: CDC25B

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Figure 1. Western blot analysis of Cdc25b using anti-Cdc25b antibody (A01899). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cdc25b antigen affinity purified polyclonal antibody (Catalog # A01899) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cdc25b at approximately 65 kDa. The expected band size for Cdc25b is at 65 kDa.
Product group Antibodies
Boster Bio
Anti-Cdc25b Antibody Picoband(r)A01899
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityMouse, Rat
TargetCdc25b
  • SizePrice
Product group Antibodies
Biorbyt
Cdc25B Rabbit monoclonal antibodyORB2298075
ApplicationsImmunoPrecipitation, Western Blot
ReactivityHuman
TargetCDC25B
  • SizePrice
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CDC25A/B/C Polyclonal Antibody, Unconjugated (bs-10579R) at 1:400 overnight at 4°C, DAB staining.
Product group Antibodies
Bioss Antibodies
CDC25A, CDC25B and CDC25C Polyclonal AntibodyBS-10579R
ApplicationsImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityBovine, Canine, Human, Mouse, Porcine, Rat
TargetCDC25C
  • SizePrice
Product group Antibodies
Abbexa
Cell Division Cycle 25B (CDC25B) AntibodyABX033268
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
Product group Antibodies
Abbexa
Cell Division Cycle 25B (CDC25B) AntibodyABX033270
ApplicationsWestern Blot, ELISA
  • SizePrice
Product group Antibodies
Abbexa
Cell Division Cycle 25B (CDC25B) AntibodyABX317871
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
  • SizePrice
Product group Antibodies
Abbexa
Cell Division Cycle 25B (CDC25B) AntibodyABX125640
ApplicationsWestern Blot
  • SizePrice
Product group Antibodies
Biorbyt
phospho-Cdc25B (Ser323) Rabbit pAbORB106117
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetCDC25B
  • SizePrice
Product group Antibodies
Biorbyt
phospho-Cdc25B (Ser186) Rabbit pAbORB4860
ApplicationsImmunoFluorescence, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetCDC25B
  • SizePrice
Product group Antibodies
Biorbyt
phospho-Cdc25B (Ser353) Rabbit pAbORB4862
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetCDC25B
  • SizePrice
Product group Cell Culture
Applied Biological Materials
CDC25B CRISPR Knockout 293T Cell LineT3003258
CategoryCell Culture
  • SizePrice
Formalin-fixed and paraffin embedded mouse embryo labeled with Rabbit Anti phospho-Cdc25B (Ser323)Polyclonal Antibody, Unconjugated (bs-5243R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Product group Antibodies
Bioss Antibodies
Cdc25B (Ser323) Polyclonal AntibodyBS-5243R
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetCDC25B
  • SizePrice
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