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Figure 1. Western blot analysis of Cdc25B using anti-Cdc25B antibody (PB9488). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cdc25B antigen affinity purified polyclonal antibody (Catalog # PB9488) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cdc25B at approximately 65 kDa. The expected band size for Cdc25B is at 65 kDa.
Product group Antibodies
Boster Bio
Anti-Cdc25B Antibody Picoband(r)PB9488-HRP
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetCDC25B
  • SizePrice
Figure 1. Western blot analysis of Cdc25B using anti-Cdc25B antibody (PB9488). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cdc25B antigen affinity purified polyclonal antibody (Catalog # PB9488) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cdc25B at approximately 65 kDa. The expected band size for Cdc25B is at 65 kDa.
Product group Antibodies
Boster Bio
Anti-Cdc25B Antibody Picoband(r)PB9488-IFLUOR647
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetCDC25B
  • SizePrice
Figure 1. Western blot analysis of Cdc25B using anti-Cdc25B antibody (PB9488). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cdc25B antigen affinity purified polyclonal antibody (Catalog # PB9488) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cdc25B at approximately 65 kDa. The expected band size for Cdc25B is at 65 kDa.
Product group Antibodies
Boster Bio
Anti-Cdc25B Antibody Picoband(r)PB9488-PE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetCDC25B
  • SizePrice
Figure 1. Western blot analysis of Cdc25B using anti-Cdc25B antibody (PB9488). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cdc25B antigen affinity purified polyclonal antibody (Catalog # PB9488) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cdc25B at approximately 65 kDa. The expected band size for Cdc25B is at 65 kDa.
Product group Antibodies
Boster Bio
Anti-Cdc25B Antibody Picoband(r)PB9488
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetCDC25B
  • SizePrice
Product group Antibodies
Santa Cruz Biotechnology
Cdc25B (DCS-162) ACSC-56266AC
ApplicationsImmunoPrecipitation
ReactivityHuman
TargetCDC25B
  • SizePrice
Product group Antibodies
Santa Cruz Biotechnology
Cdc25B (DCS-162) FITCSC-56266FITC
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman
TargetCDC25B
  • SizePrice
Product group Antibodies
Santa Cruz Biotechnology
Cdc25B (DCS-162) HRPSC-56266HRP
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman
TargetCDC25B
  • SizePrice
Product group Antibodies
Santa Cruz Biotechnology
Cdc25B (DCS-162) PESC-56266PE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman
TargetCDC25B
  • SizePrice
Product group Antibodies
Biorbyt
Cdc25B Polyclonal AntibodyORB1154901
ApplicationsImmunoFluorescence, Western Blot, ELISA
ReactivityHuman, Mouse, Rat
  • SizePrice
Product group Antibodies
Biorbyt
Cdc25B Polyclonal AntibodyORB1154902
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman
  • SizePrice
Product group Assays
Biomatik
Phospho-Cdc25B (Ser323) ELISA KitEKA51014
Assay Sample TypeResearchers plate their cell line of choice
ReactivityHuman, Mouse, Rat
  • SizePrice
Product group Antibodies
Biorbyt
Cdc25B Rabbit pAb, HRP conjugatedORB480309
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityBovine, Canine, Human, Mouse, Porcine, Rabbit, Rat
  • SizePrice
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