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Search results: CSB-E04595r

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Product group Genome Editing and Engineering
Santa Cruz Biotechnology
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CSB antibody [N2C1], Internal detects CSB protein by western blot analysis. A. 30 ug 294T whole cell lysate/extract. B. 30 ug A432 whole cell lysate/extract. C. 31 ug HeLa whole cell lysate/extract. D. 30 ug HepG3 whole cell lysate/extract. 5 % SDS-PAGE.
Product group Antibodies
OriGene
TargetERCC6
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using ERCC6 antibody.The picture on the right is treated with the synthesized peptide.
Product group Antibodies
OriGene
TargetERCC6
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Western blot analysis of extracts of various cell lines, using ERCC6 antibody (TA375967) at 1:1000 dilution. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% nonfat dry milk in TBST. - Detection: ECL Basic Kit . - Exposure time: 30s.
Product group Antibodies
OriGene
TargetERCC6
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TA326333 (0.3ug/ml) staining of HeLa nuclear lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Product group Antibodies
OriGene
TargetERCC6
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Host: Rabbit; Target Name: ERCC6; Sample Tissue: HepG2 Whole cell lysates; Antibody Dilution: 1.0 ug/ml
Product group Antibodies
OriGene
TargetERCC6
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Product group Antibodies
LifeSpan BioSciences
TargetERCC6
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Product group Antibodies
LifeSpan BioSciences
TargetERCC6
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Product group Genome Editing and Engineering
Santa Cruz Biotechnology
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Product group DNA / RNA / Vectors
OriGene
CategoryPlasmid / vector
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Figure 1. Western blot analysis of CSB using anti-CSB antibody (PB10060). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human Siha whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CSB antigen affinity purified polyclonal antibody (Catalog # PB10060) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CSB at approximately 170 kDa. The expected band size for CSB is at 168 kDa.
Product group Antibodies
Boster Bio
TargetERCC6
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Figure 1. Western blot analysis of CSB using anti-CSB antibody (PB10060). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human Siha whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CSB antigen affinity purified polyclonal antibody (Catalog # PB10060) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CSB at approximately 170 kDa. The expected band size for CSB is at 168 kDa.
Product group Antibodies
Boster Bio
TargetERCC6
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