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Figure 1. Western blot analysis of CSB using anti-CSB antibody (PB10060). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human Siha whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CSB antigen affinity purified polyclonal antibody (Catalog # PB10060) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CSB at approximately 170 kDa. The expected band size for CSB is at 168 kDa.
Product group Antibodies
Boster Bio
Anti-CSB/ERCC6 Antibody Picoband(r)PB10060-BIOTIN
TargetERCC6
  • SizePrice
Figure 1. Western blot analysis of CSB using anti-CSB antibody (PB10060). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human Siha whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CSB antigen affinity purified polyclonal antibody (Catalog # PB10060) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CSB at approximately 170 kDa. The expected band size for CSB is at 168 kDa.
Product group Antibodies
Boster Bio
Anti-CSB/ERCC6 Antibody Picoband(r)PB10060-CARRIER-FREE
TargetERCC6
  • SizePrice
Figure 1. Western blot analysis of CSB using anti-CSB antibody (PB10060). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human Siha whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CSB antigen affinity purified polyclonal antibody (Catalog # PB10060) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CSB at approximately 170 kDa. The expected band size for CSB is at 168 kDa.
Product group Antibodies
Boster Bio
Anti-CSB/ERCC6 Antibody Picoband(r)PB10060-CY3
TargetERCC6
  • SizePrice
Figure 1. Western blot analysis of CSB using anti-CSB antibody (PB10060). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human Siha whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CSB antigen affinity purified polyclonal antibody (Catalog # PB10060) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CSB at approximately 170 kDa. The expected band size for CSB is at 168 kDa.
Product group Antibodies
Boster Bio
Anti-CSB/ERCC6 Antibody Picoband(r)PB10060-DYLIGHT550
TargetERCC6
  • SizePrice
Figure 1. Western blot analysis of CSB using anti-CSB antibody (PB10060). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human Siha whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CSB antigen affinity purified polyclonal antibody (Catalog # PB10060) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CSB at approximately 170 kDa. The expected band size for CSB is at 168 kDa.
Product group Antibodies
Boster Bio
Anti-CSB/ERCC6 Antibody Picoband(r)PB10060-DYLIGHT488
TargetERCC6
  • SizePrice
Figure 1. Western blot analysis of CSB using anti-CSB antibody (PB10060). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human Siha whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CSB antigen affinity purified polyclonal antibody (Catalog # PB10060) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CSB at approximately 170 kDa. The expected band size for CSB is at 168 kDa.
Product group Antibodies
Boster Bio
Anti-CSB/ERCC6 Antibody Picoband(r)PB10060-DYLIGHT594
TargetERCC6
  • SizePrice
Figure 1. Western blot analysis of CSB using anti-CSB antibody (PB10060). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human Siha whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CSB antigen affinity purified polyclonal antibody (Catalog # PB10060) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CSB at approximately 170 kDa. The expected band size for CSB is at 168 kDa.
Product group Antibodies
Boster Bio
Anti-CSB/ERCC6 Antibody Picoband(r)PB10060-FITC
TargetERCC6
  • SizePrice
Figure 1. Western blot analysis of CSB using anti-CSB antibody (PB10060). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human Siha whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CSB antigen affinity purified polyclonal antibody (Catalog # PB10060) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CSB at approximately 170 kDa. The expected band size for CSB is at 168 kDa.
Product group Antibodies
Boster Bio
Anti-CSB/ERCC6 Antibody Picoband(r)PB10060-HRP
TargetERCC6
  • SizePrice
Figure 1. Western blot analysis of CSB using anti-CSB antibody (PB10060). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human Siha whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CSB antigen affinity purified polyclonal antibody (Catalog # PB10060) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CSB at approximately 170 kDa. The expected band size for CSB is at 168 kDa.
Product group Antibodies
Boster Bio
Anti-CSB/ERCC6 Antibody Picoband(r)PB10060-PE
TargetERCC6
  • SizePrice
Figure 1. Western blot analysis of CSB using anti-CSB antibody (PB10060). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human Siha whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CSB antigen affinity purified polyclonal antibody (Catalog # PB10060) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CSB at approximately 170 kDa. The expected band size for CSB is at 168 kDa.
Product group Antibodies
Boster Bio
Anti-CSB/ERCC6 Antibody Picoband(r)PB10060
TargetERCC6
  • SizePrice
Figure 1. Western blot analysis of CSB using anti-CSB antibody (PB10060). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human Siha whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CSB antigen affinity purified polyclonal antibody (Catalog # PB10060) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CSB at approximately 170 kDa. The expected band size for CSB is at 168 kDa.
Product group Antibodies
Boster Bio
Anti-CSB/ERCC6 Antibody Picoband(r)PB10060-IFLUOR647
TargetERCC6
  • SizePrice
Coomassie blue staining of purified ERCC6 protein (Cat# TP319020). The protein was produced from HEK293T cells transfected with ERCC6 cDNA clone (Cat# RC219020) using MegaTran 2.0 (Cat# TT210002).
Product group Proteins / Signaling Molecules
OriGene
CSB (ERCC6) (NM_000124) Human Recombinant ProteinTP319020
Protein IDQ03468
  • SizePrice
678910

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