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Search results: Caspase

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Product group Molecular Biology
Sino Biological
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Product group Antibodies
Reddot Biotech
ApplicationsImmunoFluorescence
ReactivityHuman, Mouse, Rat
TargetCASP14
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Product group Proteins / Signaling Molecules
MyBioSource
Protein IDQ92851
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Mouse Caspase-1 is detected in cell culture supernatants by ELISA using Caspase-1 (mouse) Matched Pair Detection Set (Prod. No AG-46B-0003). Method: Caspase-1 (mouse) was measured by ELISA in supernatants of differentiated bone marr
Product group Assays
AdipoGen Life Sciences
ReactivityMouse
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Product group Antibodies
References
Nordic-MUbio
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
Product group Molecular Biology
Sino Biological
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Product group Proteins / Signaling Molecules
MyBioSource
Protein IDP31944
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Western blot analysis of cleaved Caspase-9 Antibody expression in HeLa cell lysate treated with staurosporine (M00080-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CASP9 monoclonal antibody (Catalog # M00080-2) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CASP9
Product group Proteins / Signaling Molecules
Boster Bio
Product group Molecular Biology
Sino Biological
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Product group Molecular Biology
Sino Biological
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Product group Molecular Biology
Sino Biological
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Product group Molecular Biology
Sino Biological
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