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Search results: DHFR

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Western Blot Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate, Raji whole cell lysate, 293T whole cell lysate, Rat heart tissue, Mouse kidney tissue, Mouse liver tissue All lanes: DHFR antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 22, 16 kDa Observed band size: 22 kDa
Product group Antibodies
Cusabio
DHFR Recombinant Monoclonal AntibodyCSB-RA264582A0HU
ApplicationsImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetDHFR
  • SizePrice
Figure 1. Western blot analysis of DHFR using anti-DHFR antibody (PB9175). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: rat liver tissue lysates, Lane 4: rat kidney tissue lysates, Lane 5: mouse liver tissue lysates, Lane 6: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DHFR antigen affinity purified polyclonal antibody (Catalog # PB9175) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DHFR at approximately 21 kDa. The expected band size for DHFR is at 21 kDa.
Product group Antibodies
Boster Bio
Anti-Dihydrofolate reductase (DHFR) Antibody Picoband(r)PB9175
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetDHFR
  • SizePrice
WB analysis of THP-1 cells using GTX45554 DHFRL1 antibody at 0.2-1microg/ml.
Product group Antibodies
GeneTex
DHFRL1 antibody, InternalGTX45554
ApplicationsWestern Blot
TargetDHFR2
  • SizePrice
Product group Antibodies
RayBiotech
Anti-DHFR (N-term) Antibody102-23856
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetDHFR
  • SizePrice
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Product group Proteins / Signaling Molecules
Cusabio
Recombinant Human Dihydrofolate reductase (DHFR)CSB-EP006847HU
Protein IDP00374
  • SizePrice
Product group Antibodies
Bioss
DHFR Recombinant Antibody, APC ConjugatedBSM-61547R-APC
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
TargetDHFR
  • SizePrice
Product group Antibodies
Bioss
DHFR Recombinant Antibody, Biotin ConjugatedBSM-61547R-BIOTIN
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetDHFR
  • SizePrice
Product group Antibodies
Bioss
DHFR Recombinant Antibody, Cy3 ConjugatedBSM-61547R-CY3
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
TargetDHFR
  • SizePrice
Product group Antibodies
Bioss
DHFR Recombinant Antibody, Cy5.5 ConjugatedBSM-61547R-CY5.5
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
TargetDHFR
  • SizePrice
Product group Antibodies
Bioss
DHFR Recombinant Antibody, Cy5 ConjugatedBSM-61547R-CY5
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
TargetDHFR
  • SizePrice
Product group Antibodies
Bioss
DHFR Recombinant Antibody, Cy7 ConjugatedBSM-61547R-CY7
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
TargetDHFR
  • SizePrice
Product group Antibodies
Bioss
DHFR Recombinant Antibody, FITC ConjugatedBSM-61547R-FITC
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
TargetDHFR
  • SizePrice
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