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Search results: DHFR

73 to 84 of 1.102 products
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Product group Antibodies
Bioss
DHFR Polyclonal Antibody, RBITC ConjugatedBS-9058R-RBITC
ApplicationsImmunoFluorescence
TargetDHFR
  • SizePrice
Figure 1. Western blot analysis of DHFR using anti-DHFR antibody (M00813-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human SGC-7901 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human SW620 whole cell lysates, Lane 5: rat kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-DHFR antigen affinity purified monoclonal antibody (Catalog # M00813-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for DHFR at approximately 22KD. The expected band size for DHFR is at 22KD.
Product group Antibodies
References
Boster Bio
Anti-DHFR Antibody Picoband(r) (monoclonal, 3C8)M00813-1
ApplicationsWestern Blot, ImmunoHistoChemistry
TargetDHFR
  • SizePrice
Product group Antibodies
Bioss
DHFR Recombinant Antibody, PerCP ConjugatedBSM-61547R-PERCP
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
TargetDHFR
  • SizePrice
Product group Antibodies
Bioss
DHFR Polyclonal Antibody, PerCP ConjugatedBS-9058R-PERCP
ApplicationsImmunoFluorescence
TargetDHFR
  • SizePrice
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Product group Proteins / Signaling Molecules
Cusabio
Recombinant Human Dihydrofolate reductase (DHFR), partialCSB-EP006847HU1
Protein IDP00374
  • SizePrice
Product group Antibodies
Bioss
DHFR Recombinant Antibody, APC-Cy7 ConjugatedBSM-61547R-APC-CY7
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
TargetDHFR
  • SizePrice
Product group Antibodies
Bioss
DHFR Recombinant Antibody, PE-Cy5 ConjugatedBSM-61547R-PE-CY5
ApplicationsWestern Blot
TargetDHFR
  • SizePrice
Product group Antibodies
Bioss
DHFR Recombinant Antibody, PE-Cy7 ConjugatedBSM-61547R-PE-CY7
ApplicationsWestern Blot
TargetDHFR
  • SizePrice
Figure 1. Western blot analysis of DHFR using anti-DHFR antibody (M00813-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human SGC-7901 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human SW620 whole cell lysates, Lane 5: rat kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-DHFR antigen affinity purified monoclonal antibody (Catalog # M00813-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for DHFR at approximately 22KD. The expected band size for DHFR is at 22KD.
Product group Antibodies
Boster Bio
Anti-DHFR Antibody Picoband(r) (monoclonal, 3C8)M00813-1-BIOTIN
ApplicationsWestern Blot, ImmunoHistoChemistry
TargetDHFR
  • SizePrice
Figure 1. Western blot analysis of DHFR using anti-DHFR antibody (M00813-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human SGC-7901 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human SW620 whole cell lysates, Lane 5: rat kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-DHFR antigen affinity purified monoclonal antibody (Catalog # M00813-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for DHFR at approximately 22KD. The expected band size for DHFR is at 22KD.
Product group Antibodies
Boster Bio
Anti-DHFR Antibody Picoband(r) (monoclonal, 3C8)M00813-1-CARRIER-FREE
ApplicationsWestern Blot, ImmunoHistoChemistry
TargetDHFR
  • SizePrice
Figure 1. Western blot analysis of DHFR using anti-DHFR antibody (M00813-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human SGC-7901 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human SW620 whole cell lysates, Lane 5: rat kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-DHFR antigen affinity purified monoclonal antibody (Catalog # M00813-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for DHFR at approximately 22KD. The expected band size for DHFR is at 22KD.
Product group Antibodies
Boster Bio
Anti-DHFR Antibody Picoband(r) (monoclonal, 3C8)M00813-1-CY3
ApplicationsWestern Blot, ImmunoHistoChemistry
TargetDHFR
  • SizePrice
Figure 1. Western blot analysis of DHFR using anti-DHFR antibody (M00813-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human SGC-7901 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human SW620 whole cell lysates, Lane 5: rat kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-DHFR antigen affinity purified monoclonal antibody (Catalog # M00813-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for DHFR at approximately 22KD. The expected band size for DHFR is at 22KD.
Product group Antibodies
Boster Bio
Anti-DHFR Antibody Picoband(r) (monoclonal, 3C8)M00813-1-DYLIGHT488
ApplicationsWestern Blot, ImmunoHistoChemistry
TargetDHFR
  • SizePrice
56789

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