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Search results: DHFR

85 to 96 of 789 products
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Product group Chemicals
MedChem Express
DHFR-IN-2 [331942-46-2]HY-147661
CAS Number331942-46-2
Molecular Weight227.26
  • SizePrice
Product group Chemicals
MedChem Express
DHFR-IN-4 [2820126-49-4]HY-151159
CAS Number2820126-49-4
Estimated Purity99.19
Molecular Weight371.46
  • SizePrice
DHFR-IN-4 [2820126-49-4]
Product group Chemicals
TargetMol Chemicals
DHFR-IN-4 [2820126-49-4]T61486
Estimated Purity99.41%
Molecular Weight371.46
  • SizePrice
DHFR-IN-5 [1142407-81-5]
Product group Chemicals
TargetMol Chemicals
DHFR-IN-5 [1142407-81-5]T73328
Molecular Weight360.41
  • SizePrice
DHFR-IN-2 [331942-46-2]
Product group Chemicals
TargetMol Chemicals
DHFR-IN-2 [331942-46-2]T60286
Molecular Weight227.26
  • SizePrice
Product group Chemicals
MedChem Express
DHFR-IN-5 [1142407-81-5]HY-153007
CAS Number1142407-81-5
Estimated Purity99.27
Molecular Weight360.41
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Figure 1. Western blot analysis of DHFR using anti-DHFR antibody (PB9175). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: rat liver tissue lysates, Lane 4: rat kidney tissue lysates, Lane 5: mouse liver tissue lysates, Lane 6: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DHFR antigen affinity purified polyclonal antibody (Catalog # PB9175) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DHFR at approximately 21 kDa. The expected band size for DHFR is at 21 kDa.
Product group Antibodies
Boster Bio
Anti-DHFR Picoband AntibodyPB9175
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetDHFR
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
DHFR AntibodyLS-C192755
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetDHFR
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
DHFR AntibodyLS-C665069
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetDHFR
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
DHFR AntibodyLS-C806197
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Monkey, Mouse, Rat
TargetDHFR
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
DHFR AntibodyLS-C766050
ApplicationsWestern Blot
ReactivityHuman, Mouse
TargetDHFR
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
DHFR AntibodyLS-C830690
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetDHFR
  • SizePrice
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