More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/

Search results: DHFR

85 to 96 of 1.004 products
Sort by
Product group Antibodies
LifeSpan BioSciences
IHC-plus(tm) DHFR AntibodyLS-B14578
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetDHFR
  • SizePrice
Figure 1. Western blot analysis of DHFR using anti-DHFR antibody (M00813-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human SGC-7901 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human SW620 whole cell lysates, Lane 5: rat kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-DHFR antigen affinity purified monoclonal antibody (Catalog # M00813-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for DHFR at approximately 22KD. The expected band size for DHFR is at 22KD.
Product group Antibodies
References
Boster Bio
Anti-DHFR Antibody Picoband(r) (monoclonal, 3C8)M00813-1
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Rat
TargetDHFR
  • SizePrice
Product group Proteins / Signaling Molecules
Antibodies.com
Recombinant Mouse Dihydrofolate Reductase (DHFR) Protein (N-terminal His Tag)A62658
ApplicationsOther Application
  • SizePrice
Product group Proteins / Signaling Molecules
Antibodies.com
Recombinant Human Dihydrofolate Reductase (DHFR) Protein (N-terminal His Tag)A62749
ApplicationsOther Application
  • SizePrice
Product group Antibodies
MyBioSource
DHFR (Dihydrofolate Reductase, DHFRP1) (MaxLight 405)MBS6472751
  • SizePrice
Product group Antibodies
MyBioSource
DHFR (Dihydrofolate Reductase, DHFRP1) (MaxLight 490)MBS6472752
  • SizePrice
Product group Antibodies
MyBioSource
DHFR (Dihydrofolate Reductase, DHFRP1) (MaxLight 550)MBS6472753
  • SizePrice
Product group Antibodies
MyBioSource
DHFR (Dihydrofolate Reductase, DHFRP1) (MaxLight 650)MBS6472754
  • SizePrice
Product group Antibodies
MyBioSource
DHFR (Dihydrofolate Reductase, DHFRP1) (MaxLight 750)MBS6472755
  • SizePrice
Figure 1. Western blot analysis of DHFR using anti-DHFR antibody (M00813-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human SGC-7901 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human SW620 whole cell lysates, Lane 5: rat kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-DHFR antigen affinity purified monoclonal antibody (Catalog # M00813-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for DHFR at approximately 22KD. The expected band size for DHFR is at 22KD.
Product group Antibodies
Boster Bio
Anti-DHFR Antibody Picoband(r) (monoclonal, 3C8)M00813-1-BIOTIN
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Rat
TargetDHFR
  • SizePrice
Figure 1. Western blot analysis of DHFR using anti-DHFR antibody (M00813-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human SGC-7901 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human SW620 whole cell lysates, Lane 5: rat kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-DHFR antigen affinity purified monoclonal antibody (Catalog # M00813-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for DHFR at approximately 22KD. The expected band size for DHFR is at 22KD.
Product group Antibodies
Boster Bio
Anti-DHFR Antibody Picoband(r) (monoclonal, 3C8)M00813-1-CARRIER-FREE
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Rat
TargetDHFR
  • SizePrice
Figure 1. Western blot analysis of DHFR using anti-DHFR antibody (M00813-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human SGC-7901 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human SW620 whole cell lysates, Lane 5: rat kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-DHFR antigen affinity purified monoclonal antibody (Catalog # M00813-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for DHFR at approximately 22KD. The expected band size for DHFR is at 22KD.
Product group Antibodies
Boster Bio
Anti-DHFR Antibody Picoband(r) (monoclonal, 3C8)M00813-1-CY3
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Rat
TargetDHFR
  • SizePrice
678910

Didn't find what you were looking for?

Search through our product groups to find the right product

Back to overview
AntibodiesChemicalsProteins / Signaling MoleculesAssaysDNA / RNA / VectorsCell CultureGenome Editing and EngineeringMolecular BiologyLysates / ExtractsExpressionLabware / AccesoriesBody ProductsTechnique Specific ProductsArrays / SlidesDiagnosticBiologicalsPromotions