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Search results: ERK2 MAPK1

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Western blot analysis of anti-MAPK1 Antibody (Center) Pab (RB11609) in Hela, 293, and HepG2 cell line lysates. MAPK1 Antibody (Center) (arrow) was detected using the purified Pab.
Product group Antibodies
OriGene
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
ReactivityBovine, Human, Mouse, Rat, Xenopus
TargetMAPK1
  • SizePrice
TA302982 (0.5ug/ml) staining of HepG2 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Product group Antibodies
OriGene
ApplicationsWestern Blot
ReactivityBovine, Canine, Human, Mouse, Rat
TargetMAPK1
  • SizePrice
Western blot analysis on JK cell lysate using MAPK1/3 Antibody
Product group Antibodies
OriGene
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetMAPK1
  • SizePrice
Western blot analysis of anti-ERK2 C-term Pab (Cat. #TA324942) in UV-treated HL-60 cell lysate. ERK2 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Product group Antibodies
OriGene
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetMAPK1
  • SizePrice
Western blot analysis of ERK2 in (1) A431 cell lysate; (2) HeLa cell lysate.
Product group Antibodies
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetMAPK1
  • SizePrice
Western blot analysis of extracts from normal (control) and ERK2 knockout (KO) HeLa cells, using ERK2 antibody (TA378290) at 1:1000 dilution. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% nonfat dry milk in TBST. - Detection: ECL Enhanced Kit . - Exposure time: 180s.
Product group Antibodies
OriGene
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetMAPK1
  • SizePrice
Product group Antibodies
OriGene
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetMAPK1
  • SizePrice
Western blot analysis of extracts from NIH-3T3 cells, using MAPK1/3 (Ab-205/222) Antibody.The lane on the right is treated with the synthesized peptide.
Product group Antibodies
OriGene
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetMAPK1
  • SizePrice
Western blot analysis of extracts of various cell lines, using ERK1/ERK2 antibody (TA378291) at 1:1000 dilution. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% nonfat dry milk in TBST. - Detection: ECL Basic Kit . - Exposure time: 90s.
Product group Antibodies
OriGene
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetMAPK1
  • SizePrice
IHC: Human breast carcinoma is stained by Rabbit anti-ERK2 antibody at 1:200 for 10 min, RT. (Staining of formalin-fixed tissues requires boiling tissue section in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
Product group Antibodies
OriGene
ApplicationsImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetMAPK1
  • SizePrice
Western blot analysis of extracts of various cell lines, using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 antibody (TA378294) at 1:1000 dilution.Jurkat cells and NIH/3T3 cells and C6 cells were treated by PMA/TPA (200 nM) at 37°C for 30 minutes after serum-starvation overnight. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% nonfat dry milk in TBST. - Detection: ECL Enhanced Kit . - Exposure time: 180s.
Product group Antibodies
OriGene
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetMAPK1
  • SizePrice
Western blot analysis of extracts of Jurkat cells, using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (TA378295) at 1:1000 dilution or ERK1 / ERK2 antibody (A16686).Jurkat cells were treated by PMA/TPA (200 nM) at 37°C for 10 minutes. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% BSA. - Detection: ECL Basic Kit . - Exposure time: 10s.
Product group Antibodies
OriGene
ApplicationsWestern Blot
ReactivityHuman, Mouse
TargetMAPK1
  • SizePrice