Bio-Connect

Search results: ERK2 MAPK1

37 to 48 of 2.504 products
Sort by
Western blot analysis of ERK2 in (1) A431 cell lysate; (2) HeLa cell lysate.
Product group Antibodies
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetMAPK1
  • SizePrice
Western blot analysis of extracts from normal (control) and ERK2 knockout (KO) HeLa cells, using ERK2 antibody (TA378290) at 1:1000 dilution. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% nonfat dry milk in TBST. - Detection: ECL Enhanced Kit . - Exposure time: 180s.
Product group Antibodies
OriGene
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetMAPK1
  • SizePrice
Product group Antibodies
OriGene
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetMAPK1
  • SizePrice
Western blot analysis of extracts from NIH-3T3 cells, using MAPK1/3 (Ab-205/222) Antibody.The lane on the right is treated with the synthesized peptide.
Product group Antibodies
OriGene
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetMAPK1
  • SizePrice
IHC: Human breast carcinoma is stained by Rabbit anti-ERK2 antibody at 1:200 for 10 min, RT. (Staining of formalin-fixed tissues requires boiling tissue section in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
Product group Antibodies
OriGene
ApplicationsImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetMAPK1
  • SizePrice
Western blot analysis of extracts of various cell lines, using ERK1/ERK2 antibody (TA378291) at 1:1000 dilution. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% nonfat dry milk in TBST. - Detection: ECL Basic Kit . - Exposure time: 90s.
Product group Antibodies
OriGene
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetMAPK1
  • SizePrice
Western blot analysis of extracts of various cell lines, using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 antibody (TA378294) at 1:1000 dilution.Jurkat cells and NIH/3T3 cells and C6 cells were treated by PMA/TPA (200 nM) at 37°C for 30 minutes after serum-starvation overnight. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% nonfat dry milk in TBST. - Detection: ECL Enhanced Kit . - Exposure time: 180s.
Product group Antibodies
OriGene
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetMAPK1
  • SizePrice
Western blot analysis of extracts from Jurkat cells and 293 cells, using p42 MAPK antibody.The lane on the right is treated with the synthesized peptide.
Product group Antibodies
OriGene
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetMAPK1
  • SizePrice
Western blot (WB) analysis of ERK 1/2 (R172) polyclonal antibody at 1:1000 dillution Lane1:Hela whole cell lysate(40microg) Lane2:NIH-3T3 whole cell lysate(40microg) Lane3:H9C2 whole cell lysate(40microg)
Product group Antibodies
OriGene
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetMAPK1
  • SizePrice
WB: The cell lysate derived from Hela (lane 1), 3T3 (lane 2) were immunoprobed by Rabbit anti-p38 antibody at 1:500. An immunoreactive band is observed around ~38kDa.
Product group Antibodies
OriGene
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetMAPK1
  • SizePrice
WB: The whole cell lysate derived from UV treated 3T3 was separated in 10% SDS-PAGE, transferred onto NC membrane, and immunoblotted by Rabbit anti-ERK1/2 (pT202/pY204) antibody at 1:500. A dual immunoreactive band around ~42/44 kDa is observed.
Product group Antibodies
OriGene
ApplicationsWestern Blot
ReactivityChicken, Human, Mouse, Rat
TargetMAPK1
  • SizePrice
WB: The whole cell lysate derived from 3T3 was separated in 10% SDS-PAGE, transferred onto NC membrane, and immunoblotted by Rabbit anti-ERK1/2 (Paired T202/Y204) antibody at 1:500. A dual immunoreactive band around ~42/44 kDa is observed.
Product group Antibodies
OriGene
ApplicationsWestern Blot
ReactivityCanine, Chicken, Human, Mouse, Rat
TargetMAPK1
  • SizePrice