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Search results: FGF23

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Product group Antibodies
RayBiotech
ApplicationsWestern Blot
TargetFGF23
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Product group Antibodies
RayBiotech
ApplicationsWestern Blot
TargetFGF23
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Anti-FGF23 Antibody
Product group Antibodies
Antibodies.com
ApplicationsImmunoFluorescence, Western Blot
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Product group Antibodies
Antibodies.com
ApplicationsWestern Blot, ImmunoHistoChemistry
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Anti-FGF23 Antibody
Product group Antibodies
Antibodies.com
ApplicationsImmunoFluorescence, ELISA
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Lane 1: Rat Bone lysates; Lane 2: Rat Cerebrum lysates probed with FGF23 Polyclonal Antibody, Unconjugated (bs-5768R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Product group Antibodies
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetFGF23
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Product group Proteins / Signaling Molecules
Applied Biological Materials
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Product group Proteins / Signaling Molecules
Applied Biological Materials
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Figure 1. Western blot analysis of FGF23 using anti-FGF23 antibody (A00478-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat testis tissue lysate, Lane 2: rat PC-12 whole cell lysate, Lane 3: mouse testis tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGF23 antigen affinity purified polyclonal antibody (Catalog # A00478-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FGF23 at approximately 35KD. The expected band size for FGF23 is at 28KD.
Product group Antibodies
References
Boster Bio
ApplicationsWestern Blot, ELISA
TargetFgf23
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Product group Proteins / Signaling Molecules
Antibodies.com
ApplicationsFunctional Assay
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Product group Molecular Biology
MedChem Express
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Product group Antibodies
RayBiotech
ApplicationsELISA
TargetFGF23
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