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Search results: FGF23

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Human FGF23(Fibroblast Growth Factor 23) ELISA Kit
Product group Assays
Elabscience
Assay Sample TypeSerum, Plasma, Biological Fluids
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Porcine FGF23 ELISA Kit
Product group Assays
Antibodies.com
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Canine FGF23 ELISA Kit
Product group Assays
Antibodies.com
  • SizePrice
Product group Antibodies
Cusabio
ApplicationsELISA
ReactivityRat
TargetFgf23
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Product group Antibodies
Cusabio
ReactivityRat
TargetFgf23
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Product group Antibodies
Cusabio
ApplicationsELISA
ReactivityRat
TargetFgf23
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Product group Antibodies
Cusabio
ApplicationsELISA
ReactivityHuman
TargetFGF23
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Product group Antibodies
Cusabio
ReactivityHuman
TargetFGF23
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Product group Antibodies
Cusabio
ApplicationsELISA
ReactivityHuman
TargetFGF23
  • SizePrice
Figure 1. Western blot analysis of FGF 23 using anti-FGF 23 antibody (PB9868). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGF 23 antigen affinity purified polyclonal antibody (Catalog # PB9868) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FGF 23 at approximately 34 kDa. The expected band size for FGF 23 is at 28 kDa.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
TargetFgf23
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Figure 1. Western blot analysis of FGF 23 using anti-FGF 23 antibody (PB9868). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGF 23 antigen affinity purified polyclonal antibody (Catalog # PB9868) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FGF 23 at approximately 34 kDa. The expected band size for FGF 23 is at 28 kDa.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
TargetFgf23
  • SizePrice
Figure 1. Western blot analysis of FGF 23 using anti-FGF 23 antibody (PB9868). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGF 23 antigen affinity purified polyclonal antibody (Catalog # PB9868) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FGF 23 at approximately 34 kDa. The expected band size for FGF 23 is at 28 kDa.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
TargetFgf23
  • SizePrice