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Search results: HEPACAM

313 to 324 of 589 products
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Product group Proteins / Signaling Molecules
Biorbyt
  • SizePrice
Product group Antibodies
Assay Genie
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman
TargetHEPACAM2
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Product group Antibodies
Bioss Antibodies
ApplicationsWestern Blot, ELISA
ReactivityCanine, Equine, Human, Mouse, Porcine, Rabbit, Rat
TargetHEPACAM2
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WB analysis of HepG2 cells using GTX46609 HEPACAM2 antibody at 0.2-1microg/ml.
Product group Antibodies
GeneTex
ApplicationsWestern Blot
ReactivityHuman
TargetHEPACAM2
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Product group Antibodies
Biomatik
ApplicationsELISA, ImmunoHistoChemistry
TargetHEPACAM2
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Product group Proteins / Signaling Molecules
MyBioSource
Protein IDQ14CZ8
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Product group Proteins / Signaling Molecules
Biosynth
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Product group Antibodies
Cusabio
ApplicationsELISA
ReactivityHuman
TargetHEPACAM2
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Product group Antibodies
Cusabio
ReactivityHuman
TargetHEPACAM2
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Product group Antibodies
Cusabio
ApplicationsELISA
ReactivityHuman
TargetHEPACAM2
  • SizePrice
Figure 1. Western blot analysis of HEPACAM2 using anti-HEPACAM2 antibody (A12313-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human SH-SY5Y whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HEPACAM2 antigen affinity purified polyclonal antibody (Catalog # A12313-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HEPACAM2 at approximately 65 kDa. The expected band size for HEPACAM2 is at 51 kDa.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetHEPACAM2
  • SizePrice
Figure 1. Western blot analysis of HEPACAM2 using anti-HEPACAM2 antibody (A12313-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human SH-SY5Y whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HEPACAM2 antigen affinity purified polyclonal antibody (Catalog # A12313-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HEPACAM2 at approximately 65 kDa. The expected band size for HEPACAM2 is at 51 kDa.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetHEPACAM2
  • SizePrice