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Search results: Histone H3/currency/EUR

673 to 684 of 38.666 products
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Product group Antibodies
Biorbyt
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetH2AZ1
  • SizePrice
Western blot analysis of HDAC4 expression in Hela lysate (M00971). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HDAC4 monoclonal antibody (Catalog # M00971) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
Product group Antibodies
Boster Bio
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Western blot analysis of HDAC2 expression in HeLa cell lysate (M00325). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HDAC2 monoclonal antibody (Catalog # M00325) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HDAC2
Product group Antibodies
Boster Bio
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
Biorbyt
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetH2AZ1
  • SizePrice
Product group Antibodies
Biorbyt
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetH2AZ1
  • SizePrice
Western blot analysis of HDAC6 expression in HeLa cell lysate (M00595). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HDAC6 monoclonal antibody (Catalog # M00595) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HDAC6
Product group Antibodies
Boster Bio
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
IHC image of CSB-RA010109A0HU diluted at 1:100 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Product group Antibodies
Cusabio
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetH3-3A
ChIP analysis of HeLa cell lysate using GTX57170 Histone H4K20me1 (monomethyl Lys20) antibody. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
Product group Antibodies
GeneTex
ApplicationsDot Blot, ImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetH4C14
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Western blot analysis of HDAC8 expression in HeLa cell lysate (M01843). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HDAC8 monoclonal antibody (Catalog # M01843) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HDAC8
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
References
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityHuman, Zebra Fish
Targeth2ax
  • SizePrice
Histone H3 (2-58), GST-tag Recombinant
Product group Proteins / Signaling Molecules
BPS Bioscience
Protein IDP68431
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Histone H4 (2-58), GST-tag Recombinant
Product group Proteins / Signaling Molecules
BPS Bioscience
Protein IDP62805
  • SizePrice