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Figure 1. Western blot analysis of IDE using anti-IDE antibody (M01358). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human U2OS whole cell lysates, Lane 4: human SH-SY5Y whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IDE antigen affinity purified monoclonal antibody (M01358) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IDE at approximately 118 kDa. The expected band size for IDE is at 118 kDa.
Product group Antibodies
Boster Bio
Anti-IDE/Insulysin Rabbit Monoclonal AntibodyM01358-30UL
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetIDE
  • SizePrice
Figure 1. Western blot analysis of IDE using anti-IDE antibody (M01358). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human U2OS whole cell lysates, Lane 4: human SH-SY5Y whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IDE antigen affinity purified monoclonal antibody (M01358) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IDE at approximately 118 kDa. The expected band size for IDE is at 118 kDa.
Product group Antibodies
Boster Bio
Anti-IDE/Insulysin Rabbit Monoclonal AntibodyM01358
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetIDE
  • SizePrice
Product group Cell Culture
Applied Biological Materials
IDE CRISPR Knockout 293T Cell LineT3008527
CategoryCell Culture
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
IDE Antibody (aa90-139)LS-C145525
ApplicationsWestern Blot
ReactivityHuman
TargetIDE
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
IDE Antibody (aa275-326)LS-C288677
ApplicationsWestern Blot
ReactivityHuman
TargetIDE
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
IDE Antibody (aa753-973)LS-C294630
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
ReactivityHuman
TargetIDE
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
IDE Antibody (aa406-435)LS-C327665
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetIDE
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
IDE Antibody (clone 1H4)LS-C767393
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman
TargetIDE
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
IDE Antibody (Preservative Free)LS-C814886
ApplicationsWestern Blot
ReactivityHuman
TargetIDE
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
IDE Antibody (aa406-435)LS-C98186
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman
TargetIDE
  • SizePrice
Product group Proteins / Signaling Molecules
LifeSpan BioSciences
IDE Antibody Blocking PeptideLS-E10675
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
IDE Antibody (N Terminus)LS-C742487
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetIDE
  • SizePrice
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