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Search results: IL17RA

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Western blot analysis in human cell line THP-1.
Product group Antibodies
Atlas Antibodies
ApplicationsWestern Blot
ReactivityHuman
TargetIL17RA
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Western blot analysis in human cell line THP-1.
Product group Antibodies
Atlas Antibodies
ApplicationsWestern Blot
ReactivityHuman
TargetIL17RA
  • SizePrice
Product group Antibodies
RayBiotech
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetIL17RA
  • SizePrice
Product group Antibodies
Antibodies.com
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse
  • SizePrice
Anti-IL17RA Antibody
Product group Antibodies
Antibodies.com
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse
  • SizePrice
Product group Antibodies
Antibodies.com
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
  • SizePrice
Anti-IL17RA Antibody
Product group Antibodies
Antibodies.com
ApplicationsImmunoHistoChemistry
ReactivityHuman
  • SizePrice
Anti-IL17RA Antibody
Product group Antibodies
Antibodies.com
ApplicationsWestern Blot
ReactivityHuman
  • SizePrice
Product group Antibodies
Antibodies.com
ApplicationsWestern Blot
  • SizePrice
Product group Technique Specific Products
Antibodies.com
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Figure 1. Western blot analysis of IL17RA using anti-IL17RA antibody (A01311-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysate,Lane 2: human HepG2 whole cell lysate,Lane 3: human MCF-7 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL17RA antigen affinity purified polyclonal antibody (Catalog # A01311-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL17RA at approximately 160KD. The expected band size for IL17RA is at 96KD.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetIL17RA
  • SizePrice
Product group Antibodies
Assay Genie
ReactivityHuman
TargetIL17RA
  • SizePrice