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Search results: IL1A

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Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with IL-1 Alpha Polyclonal Antibody, Unconjugated (bs-4946R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Product group Antibodies
Bioss Antibodies
IL-1 Alpha Polyclonal AntibodyBS-4946R
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityHuman
TargetIL1A
  • SizePrice
Raw264.7 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with IL-1 Alpha Polyclonal Antibody(bs-4947R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Product group Antibodies
Bioss Antibodies
IL-1 Alpha Polyclonal AntibodyBS-4947R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetIl1a
  • SizePrice
Product group Antibodies
Bioss Antibodies
IL-1 Alpha Propeptide Polyclonal Antibody, Biotin ConjugatedBS-10540R-BIOTIN
ApplicationsWestern Blot, ELISA
ReactivityBovine, Canine, Human, Mouse, Porcine, Rat, Sheep
TargetIL1A
  • SizePrice
Product group Antibodies
Bioss Antibodies
IL-1 Alpha Propeptide Polyclonal Antibody, HRP ConjugatedBS-10540R-HRP
ApplicationsWestern Blot, ELISA
ReactivityBovine, Canine, Human, Mouse, Porcine, Rat, Sheep
TargetIL1A
  • SizePrice
Rat liver lysates probed with IL-1 Alpha Polyclonal Antibody, Unconjugated (bs-10540R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Product group Antibodies
Bioss Antibodies
IL-1 Alpha Propeptide Polyclonal AntibodyBS-10540R
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityBovine, Canine, Human, Mouse, Porcine, Rat, Sheep
TargetIL1A
  • SizePrice
Product group Antibodies
Arigo Biolaboratories
anti-IL1 alpha antibodyARG56666
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Neutralisation/Blocking
ReactivityHuman
TargetIL1A
  • SizePrice
Product group Antibodies
Arigo Biolaboratories
anti-IL1 alpha antibodyARG56667
ApplicationsFunctional Assay, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, Neutralisation/Blocking
ReactivityMouse
TargetIl1a
  • SizePrice
Product group Antibodies
Arigo Biolaboratories
anti-IL1 alpha antibodyARG56668
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, Neutralisation/Blocking
ReactivityRat
TargetIl1a
  • SizePrice
Product group Antibodies
Arigo Biolaboratories
anti-IL1 alpha antibody (Biotin)ARG56776
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetIL1A
  • SizePrice
Product group Antibodies
Arigo Biolaboratories
anti-IL1 alpha antibody (Biotin)ARG56777
ApplicationsWestern Blot, ELISA
ReactivityMouse
TargetIl1a
  • SizePrice
Product group Antibodies
Arigo Biolaboratories
anti-IL1 alpha antibody (Biotin)ARG56778
ApplicationsWestern Blot, ELISA
ReactivityRat
TargetIl1a
  • SizePrice
Product group Antibodies
Santa Cruz Biotechnology
IL-1alpha (ALF-161)SC-12741
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetIl1a
  • SizePrice
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