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Gel: 12%SDS-PAGE Lysate: 40 microg Lane: PC3 cell lysate Primary antibody: TA370300 (ISG15 Antibody) at dilution 1/350 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 10 seconds

Product group Antibodies

ISG15 Rabbit Polyclonal AntibodyTA370300

ApplicationsWestern Blot

ReactivityHuman

TargetISG15

SizePrice

Western blot analysis of extracts of various cell lines, using ISG15 antibody (TA377587) at 1:1000 dilution.Jurkat cells were treated by IFN-alpha (100 ng/ml) at 37°C for 30 minutes after serum-starvation overnight. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% nonfat dry milk in TBST. - Detection: ECL Basic Kit . - Exposure time: 30s.

Product group Antibodies

ISG15 Rabbit Polyclonal AntibodyTA377587

ApplicationsWestern Blot, ImmunoHistoChemistry

ReactivityHuman, Rat

TargetISG15

SizePrice

$Immunoblot of hISG15 fusion protein. Anti-hISG15 antibody, generated by immunization with recombinant human ISG15, was tested by immunoblot against a hISG15-GFP fusion protein produced in E.coli cell lysate soluble fraction. Dilution of the antibody between 1:200 and 1:1,000 showed strong reactivity specifically with hISG15 and ISG15 coupled proteins. Free hISG15 is indicated by the arrowhead. In this blot the antibody was used at a 1:200 dilution incubated overnight at 4°C in 5% non-fat dry milk in TTBS. Detection occurred using a 1:2000 dilution of HRPlabeled Donkey anti-Rabbit IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche). Other detection systems will yield similar results. Data contributed by M.Malakhov, personal$