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Search results: JUP

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Product group Antibodies
Abnova
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Product group Assays
MyBioSource
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Product group Proteins / Signaling Molecules
Abnova
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Product group Proteins / Signaling Molecules
Abnova
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Product group Proteins / Signaling Molecules
Abnova
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Product group Proteins / Signaling Molecules
Abnova
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Western blot analysis of Catenin gamma expression in (1) A431 cell lysate; (2) NIH 3T3 cell lysate; (3) C6 cell lysate.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetJUP
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Figure 1. Western blot analysis of JUP using anti-JUP antibody (PA1117-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human T47D whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-JUP antigen affinity purified polyclonal antibody (Catalog # PA1117-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for JUP at approximately 82 kDa. The expected band size for JUP is at 82 kDa.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHamster, Human
TargetJUP
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Figure 1. Western blot analysis of gamma Catenin using anti-gamma Catenin antibody (PB9185). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human T47D whole cell lysates, Lane 4: rat heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-gamma Catenin antigen affinity purified polyclonal antibody (Catalog # PB9185) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for gamma Catenin at approximately 82 kDa. The expected band size for gamma Catenin is at 82 kDa.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
ReactivityHuman, Mouse, Rat
TargetJUP
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Product group Cell Culture
Abnova
CategoryCell Culture
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Product group Antibodies
Abnova
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Product group Antibodies
Biorbyt
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rabbit, Rat
TargetJUP
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