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Search results: JUP

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Product group Antibodies
Biorbyt
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rabbit, Rat
TargetJUP
  • SizePrice
Figure 1. Western blot analysis of Catenin gamma(Plakoglobin) using anti-Catenin gamma(Plakoglobin) antibody (MA1012). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: human CACO-2 whole cell lysates, Lane 6: human K562 whole cell lysates, Lane 7: human 293T whole cell lysates, Lane 8: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Catenin gamma(Plakoglobin) antigen affinity purified monoclonal antibody (Catalog # MA1012) at 1 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Catenin gamma(Plakoglobin) at approximately 82 kDa. The expected band size for Catenin gamma(Plakoglobin) is at 82 kDa.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityBovine, Human, Monkey
TargetJUP
  • SizePrice
Product group Antibodies
Abnova
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Product group Antibodies
Wuhan Fine Biotech
JUP antibodyFNAB03334
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetJUP
  • SizePrice