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Search results: LOX CRISPR

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Figure 1. Western blot analysis of LOX-1/OLR1 using anti-LOX-1/OLR1 antibody (A00760-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LOX-1/OLR1 antigen affinity purified polyclonal antibody (Catalog # A00760-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LOX-1/OLR1 at approximately 38KD. The expected band size for LOX-1/OLR1 is at 31KD.
Product group Antibodies
Boster Bio
Anti-LOX-1/OLR1 Antibody Picoband(r)A00760-1-FITC
ApplicationsWestern Blot
TargetOLR1
  • SizePrice
Figure 1. Western blot analysis of LOX-1/OLR1 using anti-LOX-1/OLR1 antibody (A00760-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LOX-1/OLR1 antigen affinity purified polyclonal antibody (Catalog # A00760-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LOX-1/OLR1 at approximately 38KD. The expected band size for LOX-1/OLR1 is at 31KD.
Product group Antibodies
Boster Bio
Anti-LOX-1/OLR1 Antibody Picoband(r)A00760-1-HRP
ApplicationsWestern Blot
TargetOLR1
  • SizePrice
Figure 1. Western blot analysis of LOX-1/OLR1 using anti-LOX-1/OLR1 antibody (A00760-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LOX-1/OLR1 antigen affinity purified polyclonal antibody (Catalog # A00760-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LOX-1/OLR1 at approximately 38KD. The expected band size for LOX-1/OLR1 is at 31KD.
Product group Antibodies
Boster Bio
Anti-LOX-1/OLR1 Antibody Picoband(r)A00760-1-IFLUOR647
ApplicationsWestern Blot
TargetOLR1
  • SizePrice
Figure 1. Western blot analysis of LOX-1/OLR1 using anti-LOX-1/OLR1 antibody (A00760-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LOX-1/OLR1 antigen affinity purified polyclonal antibody (Catalog # A00760-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LOX-1/OLR1 at approximately 38KD. The expected band size for LOX-1/OLR1 is at 31KD.
Product group Antibodies
Boster Bio
Anti-LOX-1/OLR1 Antibody Picoband(r)A00760-1-PE
ApplicationsWestern Blot
TargetOLR1
  • SizePrice
Product group DNA / RNA / Vectors
Sino Biological
Human, LOX cDNA ORF Clone, C-GFPSpark(r) tagHG17796-ACG
CategoryDNA / RNA / Vectors
  • SizePrice
Product group DNA / RNA / Vectors
Sino Biological
Human, LOX cDNA ORF Clone, C-OFPSpark(r) tagHG17796-ACR
CategoryDNA / RNA / Vectors
  • SizePrice
Product group DNA / RNA / Vectors
Sino Biological
Rhesus, LOX-1 cDNA ORF Clone, C-GFPSpark(r) tagCG90155-ACG
CategoryDNA / RNA / Vectors
  • SizePrice
Product group DNA / RNA / Vectors
Sino Biological
Rhesus, LOX-1 cDNA ORF Clone, C-OFPSpark(r) tagCG90155-ACR
CategoryDNA / RNA / Vectors
  • SizePrice
Product group DNA / RNA / Vectors
Sino Biological
Human, LOX-1 cDNA ORF Clone, C-GFPSpark(r) tagHG10585-ACG
CategoryDNA / RNA / Vectors
  • SizePrice
Product group DNA / RNA / Vectors
Sino Biological
Human, LOX-1 cDNA ORF Clone, C-OFPSpark(r) tagHG10585-ACR
CategoryDNA / RNA / Vectors
  • SizePrice
Product group DNA / RNA / Vectors
Sino Biological
Human, LOX cDNA ORF Clone, C-DYKDDDDK (Flag(r)) tagHG17796-CF
CategoryDNA / RNA / Vectors
  • SizePrice
Product group DNA / RNA / Vectors
Sino Biological
Human, LOX cDNA ORF Clone, N-DYKDDDDK (Flag(r)) tagHG17796-NF
CategoryDNA / RNA / Vectors
  • SizePrice
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