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Search results: MAP22K3 MEK3 MKK3

205 to 216 of 990 products
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Product group Assays
Biomatik
Phospho-Mkk3 (Thr222) ELISA KitEKA51629
Assay Sample TypeResearchers plate their cell line of choice
ReactivityHuman, Mouse, Rat
  • SizePrice
Product group Molecular Biology
Sino Biological
Human, MEK3/MAP2K3 cDNA ORF Clone, untaggedHG18688-UT
  • SizePrice
Product group Molecular Biology
Sino Biological
Mouse, MEK3/MAP2K3 cDNA ORF Clone, untaggedMG52648-UT
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Flow cytometric analysis of HeLa cells with MEK3 (4C1) Monoclonal Antibody (bsm-52658R) at a 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Product group Antibodies
Bioss
MEK3 (4C1) Monoclonal AntibodyBSM-52658R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetMAP2K3
  • SizePrice
Figure 2. Immunocytochemistry staining of MAP2K3 using Anti-MEK3 MAP2K3 Antibody (A02916-1). ICC staining MEK3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde
Product group Antibodies
Boster Bio
Anti-MEK3 MAP2K3 AntibodyA02916-1
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetMAP2K3
  • SizePrice
Product group Antibodies
MedChem Express
MEK3 Antibody (YA3214, Rabbit)HY-P83469
TargetMAP2K3
  • SizePrice
Product group Antibodies
MedChem Express
MEK3 Antibody (YA4038, Mouse)HY-P84341
TargetMAP2K3
  • SizePrice
Product group Antibodies
MedChem Express
MEK3 Antibody (YA6048, Rabbit)HY-P86356
TargetMAP2K3
  • SizePrice
Figure 1. Western blot analysis of MEK3 using anti-MEK3 antibody (PB9763). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: rat kidney tissue lysates, Lane 3: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MEK3 antigen affinity purified polyclonal antibody (Catalog # PB9763) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MEK3 at approximately 40 kDa. The expected band size for MEK3 is at 40 kDa.
Product group Antibodies
References
Boster Bio
Anti-MEK3 Picoband AntibodyPB9763
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityBovine, Human, Mouse, Rat
TargetMAP2K3
  • SizePrice
Figure 2. IP validation of MAP2K6 using Anti-MKK3/6 MAP2K6 Monoclonal Antibody (M02011-2). Immunoprecipitation analysis of Hela cell lysates using MKK3/6 mouse mAb.
Product group Antibodies
Boster Bio
Anti-MKK3/6 MAP2K6 Monoclonal AntibodyM02011-2
ApplicationsImmunoPrecipitation, Western Blot
ReactivityHuman, Rat
TargetMAP2K6
  • SizePrice
Boster Kit Box
Product group Assays
Boster Bio
MKK3 Colorimetric Cell-Based ELISA KitEKC1371
ReactivityHuman, Mouse, Rat
  • SizePrice
Figure 2. IHC analysis of MAP2K3 using anti-MAP2K3 antibody (A02916). MAP2K3 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MAP2K3 Antibody (A02916) overnight at 4°C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
References
Boster Bio
Anti-MKK3 (Ab-189) MAP2K3 AntibodyA02916
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetMAP2K3
  • SizePrice
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