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Search results: MAP22K3 MEK3 MKK3

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Paraformaldehyde-fixed, paraffin embedded Rat stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with MEK3/MAP2K3 Polyclonal Antibody, Unconjugated (bs-1689R) at 1:400 overnight at 4°C, DAB staining.
Product group Antibodies
Bioss
MEK3 Polyclonal Antibodybs-1689R
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetMAP2K3
  • SizePrice
Anti-MEK3 Antibody
Product group Antibodies
Antibodies.com
Anti-MEK3 AntibodyA15852
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
  • SizePrice
Product group Antibodies
Bioss
MEK3 Recombinant AntibodyBSM-60662R
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetMAP2K3
  • SizePrice
Anti-MEK3 Antibody
Product group Antibodies
Antibodies.com
Anti-MEK3 AntibodyA48506
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
  • SizePrice
Product group Antibodies
Abbexa
OsMKK3 AntibodyABX018503
ApplicationsWestern Blot
  • SizePrice
Anti-MEK3 antibody, PA1377-1, All Western blotting All lanes: Anti-MAP2K3(PA1377-1) at 0.5ug/ml Lane 1: Rat Skeletal Muscle Tissue Lysate at 40ug Lane 2: Rat Kidney Tissue Lysate at 40ug Lane 3: Rat Spleen Tissue Lysate at 40ug Lane 4: HELA Whole Cell Lysate at 40ug Lane 5: A549 Whole Cell Lysate at 40ug Lane 6: JURKAT Whole Cell Lysate at 40ug Lane 7: CEM Whole Cell Lysate at 40ug Predicted bind size: 39KD Observed bind size: 39KD
Product group Antibodies
Boster Bio
Anti-MEK3 AntibodyPA1377-1
ApplicationsWestern Blot
ReactivityHamster, Human, Mouse, Rat
TargetMAP2K3
  • SizePrice
Figure 1. Western blot analysis of MAP2K3 using anti-MAP2K3 antibody (PA1377). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human CACO-2 whole cell lysates, Lane 3: human HEK293 whole cell lysates, Lane 4: human HEPG2 whole cell lysates, Lane 5: human PC-3 whole cell lysates, Lane 6: human HELA whole cell lysates, Lane 7: human THP-1 whole cell lysates, Lane 8: rat brain tissue lysates, Lane 9: rat heart tissue lysates, Lane 10: rat NRK whole cell lysates, Lane 11: mouse Neuro-2a whole cell lysates, Lane 12: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP2K3 antigen affinity purified polyclonal antibody (Catalog # PA1377) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAP2K3 at approximately 39KD. The expected band size for MAP2K3 is at 39KD.
Product group Antibodies
Boster Bio
Anti-MEK3 AntibodyPA1377
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHamster, Human, Mouse, Rat
TargetMAP2K3
  • SizePrice
Anti-MKK3 (phospho Ser189) Antibody
Product group Antibodies
Antibodies.com
Anti-MKK3 (phospho Ser189) AntibodyA94431
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
  • SizePrice
Anti-MKK3 (Ab-189) Antibody
Product group Antibodies
Antibodies.com
Anti-MKK3 (Ab-189) AntibodyA34687
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse
  • SizePrice
MKK3 Cell Based ELISA Kit
Product group Assays
Antibodies.com
MKK3 Cell Based ELISA KitA103247
Assay Sample TypeAdherent cells and suspension cells.
ReactivityHuman, Mouse, Rat
  • SizePrice
Anti-MKK3 (Phospho-Ser189) Antibody
Product group Antibodies
Antibodies.com
Anti-MKK3 (Phospho-Ser189) AntibodyA50176
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
  • SizePrice
Product group Assays
Biomatik
Phospho-Mkk3 (Ser189) ELISA KitEKA51509
Assay Sample TypeResearchers plate their cell line of choice
ReactivityHuman, Mouse, Rat
  • SizePrice
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