More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/

Search results: MAP2K1 MEK1

313 to 324 of 3.141 products
Sort by
Figure 2. Immunohistochemistry validation of MAP2K1 using Anti-MEK1 (phospho-T292) MAP2K1 Antibody (A00292T292). Immunohistochemistry (IHC) analyzes of p-MEK1 (T292) pAb in paraffin-embedded human breast carcinoma tissue at 1:100. For more protocol information of IHC
Product group Antibodies
Boster Bio
Anti-MEK1 (phospho-T292) MAP2K1 AntibodyA00292T292
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetMAP2K1
  • SizePrice
Western blot of human T47D cells showing specific immunolabeling of the ~45 kDa MEK 1 protein phosphorylated at Thr386 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (gamma-Ptase, 1200 units for 30 min).
Product group Antibodies
Boster Bio
Anti-Phospho-MEK1 (Thr386) MAP2K1 AntibodyP00292-2
ApplicationsWestern Blot
ReactivityBovine, Canine, Chicken, Human, Mouse, Primate, Rat, Xenopus
TargetMAP2K1
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
MAP2K1 / MKK1 / MEK1 Antibody (C-Terminus, clone 13B6.G12)LS-C744857
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetMAP2K1
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
MAP2K1 / MKK1 / MEK1 Antibody (C-Terminus, clone 13B6.G12)LS-C744859
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetMAP2K1
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
IHC-plus(tm) MAP2K1 / MKK1 / MEK1 Antibody (N-Terminus)LS-B3271
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat, Xenopus
TargetMAP2K1
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
IHC-plus(tm) MAP2K1 / MKK1 / MEK1 Antibody (aa264-313)LS-B7631
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetMAP2K1
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
IHC-plus(tm) MAP2K1 / MKK1 / MEK1 Antibody (aa252-301)LS-B7632
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetMAP2K1
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
MAP2K1 / MKK1 / MEK1 Antibody (aa269-296, Azide-free, HRP)LS-C223162
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetMAP2K1
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
MAP2K1 / MKK1 / MEK1 Antibody (phospho-Ser222, Azide-free, HRP)LS-C225873
ApplicationsDot Blot, ELISA
ReactivityHuman
TargetMAP2K1
  • SizePrice
Figure 1. Western blot analysis of MEK1-2 using anti-MEK1-2 antibody (M00292-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human U2OS whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat lung tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MEK1-2 antigen affinity purified monoclonal antibody (Catalog # M00292-1) at 1:5000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MEK1-2 at approximately 43 kDa. The expected band size for MEK1-2 is at 43 kDa.
Product group Antibodies
Boster Bio
Anti-MEK1/2 MAP2K1 Rabbit Monoclonal AntibodyM00292-1-30UL
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetMAP2K1
  • SizePrice
Figure 1. Western blot analysis of MEK1-2 using anti-MEK1-2 antibody (M00292-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human U2OS whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat lung tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MEK1-2 antigen affinity purified monoclonal antibody (Catalog # M00292-1) at 1:5000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MEK1-2 at approximately 43 kDa. The expected band size for MEK1-2 is at 43 kDa.
Product group Antibodies
References
Boster Bio
Anti-MEK1/2 MAP2K1 Rabbit Monoclonal AntibodyM00292-1
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetMAP2K1
  • SizePrice
Product group Antibodies
Biorbyt
Anti-MEK1/MAP2K1 AntibodyORB27588
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetMAP2K1
  • SizePrice
2526272829

Didn't find what you were looking for?

Search through our product groups to find the right product

Back to overview
AntibodiesChemicalsProteins / Signaling MoleculesAssaysDNA / RNA / VectorsCell CultureGenome Editing and EngineeringMolecular BiologyLysates / ExtractsExpressionLabware / AccesoriesBody ProductsTechnique Specific ProductsArrays / SlidesDiagnosticBiologicalsMicrobiologyPromotions