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Search results: MAP2K1 MEK1

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Western blots of recombinant wild type and mutant MEK 1 showing phospho-specific immunolabeling. Lanes 1 and 2 are wild type MEK 1; lanes 3 and 4 are mutant MEK 1 (Thr 200 Arg). MAP kinase was co-expressed in the samples run in lanes 2 and 4. MEK1 (phospho Thr292) antibody was used (GTX82657).
Product group Antibodies
GeneTex
MEK1 (phospho Thr292) antibodyGTX82657
ApplicationsWestern Blot
ReactivityHuman
TargetMAP2K1
  • SizePrice
ICC/IF analysis of methanol-fixed HeLa cells using GTX50216 MEK1 (phospho Ser221) antibody.
Product group Antibodies
GeneTex
MEK1 (phospho Ser221) antibodyGTX50216
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman
TargetMAP2K1
  • SizePrice
Western blot analysis of MEK1/2 phosphorylation expression in PMA treated 293 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
Product group Antibodies
GeneTex
MEK1/2 (phospho Ser217) antibodyGTX52336
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetMAP2K1
  • SizePrice
Western blot analysis of MEK1/2 phosphorylation expression in UV treated Jurkat whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
Product group Antibodies
GeneTex
MEK1/2 (phospho Ser221) antibodyGTX52337
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetMAP2K1
  • SizePrice
WB analysis of PMA-treated HEK293T (A), UV-treated NIH3T3 (B), C2C12 (C), PMA-treated PC12 (D), C6 (E) whole cell lysates using GTX32248 MEK1 (phospho Thr286) antibody.
Product group Antibodies
GeneTex
MEK1 (phospho Thr286) antibodyGTX32248
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetMAP2K1
  • SizePrice
ICC/IF analysis of formalin-fixed HeLa cells using GTX32249 MEK1 (phospho Thr292) antibody. Red : Primary antibody Blue : DAPI Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes
Product group Antibodies
GeneTex
MEK1 (phospho Thr292) antibodyGTX32249
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman
TargetMAP2K1
  • SizePrice
Western blot analysis of MEK1 expression in A431 cell lysate (M00292). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP2K1 monoclonal antibody (Catalog # M00292) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAP2K1
Product group Antibodies
Boster Bio
Anti-MEK1 MAP2K1 Rabbit Monoclonal AntibodyM00292
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetMAP2K1
Western blot analysis of MEK1 expression in (1) A431 cell lysate;(2) HeLa cell lysate (M00292-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP2K1 monoclonal antibody (Catalog # M00292-2) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAP2K1
Product group Antibodies
Boster Bio
Anti-MEK1 MAP2K1 Rabbit Monoclonal AntibodyM00292-2
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetMAP2K1
Product group Molecular Biology
OriGene
MEK1 (MAP2K1) Human siRNA Oligo Duplex (Locus ID 5604)SR321446
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Product group DNA / RNA / Vectors
OriGene
MEK1 (MAP2K1) (NM_002755) Human Tagged ORF Clone Lentiviral ParticleRC218460L1V
  • SizePrice
Product group DNA / RNA / Vectors
OriGene
MEK1 (MAP2K1) (NM_002755) Human Tagged ORF Clone Lentiviral ParticleRC218460L3V
  • SizePrice
Product group DNA / RNA / Vectors
OriGene
MEK1 (MAP2K1) Human shRNA Plasmid Kit (Locus ID 5604)TF320489
  • SizePrice
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