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Search results: MAP2K1 MEK1

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ICC/IF analysis of formalin-fixed K562 cells using GTX54969 MEK1/2 (phospho Ser222/Ser226) antibody. Red : Primary antibody Blue : DAPI Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes
Product group Antibodies
GeneTex
MEK1/2 (phospho Ser222/Ser226) antibodyGTX54969
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman
TargetMAP2K1
  • SizePrice
Western Blot: MEK1/2 Phosphospecific Antibody [GTX48615] - Western Blot of NIH 3T3 cell lysates showing specific immunolabeling of the ~45kDa MEK 1/2 protein phosphorylated at Ser218 and Ser222. The cells were either serum starved (Control) or incubated in the presence of serum (Serum). Immunolabeling of an additional band at ~95kDa was also observed.
Product group Antibodies
GeneTex
MEK1/2 (phospho Ser218/222) antibodyGTX48615
ApplicationsWestern Blot
ReactivityBovine, Canine, Chicken, Human, Monkey, Mouse, Rat, Xenopus, Zebra Fish
TargetMAP2K1
  • SizePrice
Product group Antibodies
GeneTex
MEK1 + MEK2 antibody [N2C2], InternalGTX121943
ApplicationsWestern Blot
ReactivityHuman
TargetMAP2K1
  • SizePrice
Immunohistochemical analysis of paraffin-embedded human kidney, using MEK1/2 Antibody(M00292-1) MAP2K1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MAP2K1 Antibody (M00292-1)overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
Boster Bio
Anti-MEK1/2 MAP2K1 Rabbit Monoclonal AntibodyM00292-1
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetMAP2K1
Product group Antibodies
GeneTex
MEK1 (phospho Ser221) antibody - Orthogonal ValidatedGTX79016
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman
TargetMAP2K1
  • SizePrice
Product group Antibodies
GeneTex
MEK1 (phospho Thr291) antibody - Orthogonal ValidatedGTX79017
ApplicationsWestern Blot
ReactivityHuman
TargetMAP2K1
  • SizePrice
IHC-P analysis of human breast carcinoma tissue using GTX50860 MEK1/MEK2 (phospho Ser217/Ser221) antibody. Left : Primary antibody Right : Primary antibody pre-incubated with the antigen specific peptide
Product group Antibodies
GeneTex
MEK1/MEK2 (phospho Ser217/Ser221) antibodyGTX50860
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman
TargetMAP2K1
  • SizePrice
WB analysis of extracts from HepG2 cells untreated or treated with PMA using GTX50313 MEK1 (phospho Thr291) antibody.
Product group Antibodies
GeneTex
MEK1 (phospho Thr291) antibody - Orthogonal ValidatedGTX50313
ApplicationsWestern Blot
ReactivityHuman
TargetMAP2K1
  • SizePrice
IHC-P analysis of human kidney tissue using GTX25612 MEK1 (phospho Thr292) antibody. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min Dilution : 1:100
Product group Antibodies
GeneTex
MEK1 (phospho Thr292) antibody - Orthogonal ValidatedGTX25612
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetMAP2K1
  • SizePrice
IHC-P analysis of human colon carcinoma tissue using GTX25613 MEK1 (phospho Ser298) antibody. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min Dilution : 1:100
Product group Antibodies
References
GeneTex
MEK1 (phospho Ser298) antibody - Orthogonal ValidatedGTX25613
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetMAP2K1
  • SizePrice
WB (peptide competition) analysis of NIH3T3 cells treated with 50ng/mL PDGF for 15 minutes (Lane 2-5) using GTX25615 MEK1 (phospho Thr386) antibody with the non-phosphopeptide corresponding to the phosphopeptide immunogen (Lane 3), a generic phosphothreonine-containing peptide (Lane 4), or the phosphopeptide immunogen (Lane 5) control. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.
Product group Antibodies
GeneTex
MEK1 (phospho Thr386) antibody - Orthogonal ValidatedGTX25615
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetMAP2K1
  • SizePrice
Western blot analysis of MEK5 expression in (1) HeLa cell lysate; (2) HeLa cell treated with Nocodazole (P00292-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP2K1 monoclonal antibody (Catalog # P00292-4) overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAP2K1
Product group Antibodies
Boster Bio
Anti-Phospho-MEK1 (T292) MAP2K1 Rabbit Monoclonal AntibodyP00292-4
ApplicationsWestern Blot
ReactivityHuman
TargetMAP2K1
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