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Search results: MAP2K1 MEK1

817 to 828 of 3.169 products
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Product group Assays
Assay Genie
Human MEK1 ELISA KitHUFI00419
Assay Sample TypeSerum, Plasma
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Product group Antibodies
Assay Genie
MEK1/MEK2 Monoclonal AntibodyCAB4868
TargetMAP2K1
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Product group Antibodies
Biorbyt
MAP2K1ORB1288106
ApplicationsELISA, ImmunoHistoChemistry
TargetMAP2K1
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Western blot analysis of MEK1/2 in human brain tissue lysate with MEK1/2 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.
Product group Antibodies
OriGene
MEK1 (MAP2K1) Rabbit Polyclonal AntibodyTA349182
ApplicationsWestern Blot
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Product group Assays
Assay Genie
Rat MAP2k1 ColorStep ELISA KitAEFI02845
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Product group Lysates / Extracts
LifeSpan BioSciences
MAP2K1 / MKK1 / MEK1 Protein (Over-Expression Lysate Myc-DDK (Flag)) (Full Length)LS-G99857
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Figure 1. Western blot analysis of MEK1-2 using anti-MEK1-2 antibody (M00292-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human U2OS whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat lung tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MEK1-2 antigen affinity purified monoclonal antibody (Catalog # M00292-1) at 1:5000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MEK1-2 at approximately 43 kDa. The expected band size for MEK1-2 is at 43 kDa.
Product group Antibodies
References
Boster Bio
Anti-MEK1/2 MAP2K1 Rabbit Monoclonal AntibodyM00292-1
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetMAP2K1
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Product group Proteins / Signaling Molecules
LifeSpan BioSciences
Mouse MAP2K1 / MKK1 / MEK1 Protein (Recombinant His/GST,N-terminus) (Met1-Ile393)LS-G139937
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using MAP3K7 (Ab-187) antibody.
Product group Antibodies
Cusabio
MAP3K7 (Ab-187) AntibodyCSB-PA173975
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetMAP3K7
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Western blot analysis of extracts from HeLa cells, using MAP2K7 (Ab-271) antibody.
Product group Antibodies
Cusabio
MAP3K7 (Ab-271) AntibodyCSB-PA206068
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse
TargetMAP3K7
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Western blot analysis of extracts from HuvEc, using C-RAF (Phospho-Ser301) antibody. The lane on the right is treated with the synthesized peptide.
Product group Antibodies
Cusabio
Phospho-RAF1 (Ser301) AntibodyCSB-PA948259
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetRAF1
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Product group Antibodies
Biosynth
MAP2K1 antibody (Thr386)70R-32676
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