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Search results: MAP2K2 MEK2

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Western blot analysis of MEK2 expression in Jurkat cell lysate (M00996). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP2K2 monoclonal antibody (Catalog # M00996) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAP2K2
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetMAP2K2
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Product group Antibodies
LifeSpan BioSciences
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityCanine, Chicken, Human, Monkey, Mouse, Porcine, Rabbit, Rat
  • SizePrice
Product group Antibodies
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityCanine, Drosophila, Feline, Human
  • SizePrice
Product group Antibodies
Biorbyt
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetMAP2K2
  • SizePrice
Product group Antibodies
Biorbyt
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetMAP2K2
  • SizePrice
Product group Antibodies
MyBioSource
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Product group Antibodies
MyBioSource
  • SizePrice
Product group Antibodies
Bioss Antibodies
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityChicken, Human, Mouse, Rat
TargetMAP2K2
  • SizePrice
Product group Antibodies
Bioss Antibodies
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityChicken, Human, Mouse, Rat
TargetMAP2K2
  • SizePrice