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Search results: MAP2K4 MEK4 MKK4 SEK1

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Figure 1. Western blot analysis of MAP2K4 using anti-MAP2K4 antibody (A01725-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: human 22RV1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP2K4 antigen affinity purified polyclonal antibody (Catalog # A01725-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAP2K4 at approximately 44KD. The expected band size for MAP2K4 is at 44KD.
Product group Antibodies
Boster Bio
Anti-MEK4/MAP2K4 Antibody Picoband(r)A01725-1-PE
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetMAP2K4
  • SizePrice
Figure 1. Western blot analysis of MAP2K4 using anti-MAP2K4 antibody (A01725-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: human 22RV1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP2K4 antigen affinity purified polyclonal antibody (Catalog # A01725-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAP2K4 at approximately 44KD. The expected band size for MAP2K4 is at 44KD.
Product group Antibodies
References
Boster Bio
Anti-MEK4/MAP2K4 Antibody Picoband(r)A01725-1
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetMAP2K4
  • SizePrice
Anti-MEK4 antibody, PA2275, All Western blotting All lanes: Anti-MAP2K4(PA2275) at 0.5ug/ml Lane 1: Rat Skeletal Muscle Tissue Lysate at 40ug Lane 2: HELA Whole Cell Lysate at 40ug Lane 3: A549 Whole Cell Lysate at 40ug Lane 4: MM231 Whole Cell Lysate at 40ug Lane 5: CEM Whole Cell Lysate at 40ug Predicted bind size: 44KD Observed bind size: 44KD
Product group Antibodies
Boster Bio
Anti-MEK4/MAP2K4 Antibody Picoband(r)PA2275-10UG
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetMAP2K4
  • SizePrice
Anti-MEK4 antibody, PA2275, All Western blotting All lanes: Anti-MAP2K4(PA2275) at 0.5ug/ml Lane 1: Rat Skeletal Muscle Tissue Lysate at 40ug Lane 2: HELA Whole Cell Lysate at 40ug Lane 3: A549 Whole Cell Lysate at 40ug Lane 4: MM231 Whole Cell Lysate at 40ug Lane 5: CEM Whole Cell Lysate at 40ug Predicted bind size: 44KD Observed bind size: 44KD
Product group Antibodies
Boster Bio
Anti-MEK4/MAP2K4 Antibody Picoband(r)PA2275-APC
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetMAP2K4
  • SizePrice
Anti-MEK4 antibody, PA2275, All Western blotting All lanes: Anti-MAP2K4(PA2275) at 0.5ug/ml Lane 1: Rat Skeletal Muscle Tissue Lysate at 40ug Lane 2: HELA Whole Cell Lysate at 40ug Lane 3: A549 Whole Cell Lysate at 40ug Lane 4: MM231 Whole Cell Lysate at 40ug Lane 5: CEM Whole Cell Lysate at 40ug Predicted bind size: 44KD Observed bind size: 44KD
Product group Antibodies
Boster Bio
Anti-MEK4/MAP2K4 Antibody Picoband(r)PA2275-BIOTIN
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetMAP2K4
  • SizePrice
Anti-MEK4 antibody, PA2275, All Western blotting All lanes: Anti-MAP2K4(PA2275) at 0.5ug/ml Lane 1: Rat Skeletal Muscle Tissue Lysate at 40ug Lane 2: HELA Whole Cell Lysate at 40ug Lane 3: A549 Whole Cell Lysate at 40ug Lane 4: MM231 Whole Cell Lysate at 40ug Lane 5: CEM Whole Cell Lysate at 40ug Predicted bind size: 44KD Observed bind size: 44KD
Product group Antibodies
Boster Bio
Anti-MEK4/MAP2K4 Antibody Picoband(r)PA2275-CARRIER-FREE
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetMAP2K4
  • SizePrice
Anti-MEK4 antibody, PA2275, All Western blotting All lanes: Anti-MAP2K4(PA2275) at 0.5ug/ml Lane 1: Rat Skeletal Muscle Tissue Lysate at 40ug Lane 2: HELA Whole Cell Lysate at 40ug Lane 3: A549 Whole Cell Lysate at 40ug Lane 4: MM231 Whole Cell Lysate at 40ug Lane 5: CEM Whole Cell Lysate at 40ug Predicted bind size: 44KD Observed bind size: 44KD
Product group Antibodies
Boster Bio
Anti-MEK4/MAP2K4 Antibody Picoband(r)PA2275-CY3
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetMAP2K4
  • SizePrice
Anti-MEK4 antibody, PA2275, All Western blotting All lanes: Anti-MAP2K4(PA2275) at 0.5ug/ml Lane 1: Rat Skeletal Muscle Tissue Lysate at 40ug Lane 2: HELA Whole Cell Lysate at 40ug Lane 3: A549 Whole Cell Lysate at 40ug Lane 4: MM231 Whole Cell Lysate at 40ug Lane 5: CEM Whole Cell Lysate at 40ug Predicted bind size: 44KD Observed bind size: 44KD
Product group Antibodies
Boster Bio
Anti-MEK4/MAP2K4 Antibody Picoband(r)PA2275-DYLIGHT488
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetMAP2K4
  • SizePrice
Anti-MEK4 antibody, PA2275, All Western blotting All lanes: Anti-MAP2K4(PA2275) at 0.5ug/ml Lane 1: Rat Skeletal Muscle Tissue Lysate at 40ug Lane 2: HELA Whole Cell Lysate at 40ug Lane 3: A549 Whole Cell Lysate at 40ug Lane 4: MM231 Whole Cell Lysate at 40ug Lane 5: CEM Whole Cell Lysate at 40ug Predicted bind size: 44KD Observed bind size: 44KD
Product group Antibodies
Boster Bio
Anti-MEK4/MAP2K4 Antibody Picoband(r)PA2275-DYLIGHT550
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetMAP2K4
  • SizePrice
Anti-MEK4 antibody, PA2275, All Western blotting All lanes: Anti-MAP2K4(PA2275) at 0.5ug/ml Lane 1: Rat Skeletal Muscle Tissue Lysate at 40ug Lane 2: HELA Whole Cell Lysate at 40ug Lane 3: A549 Whole Cell Lysate at 40ug Lane 4: MM231 Whole Cell Lysate at 40ug Lane 5: CEM Whole Cell Lysate at 40ug Predicted bind size: 44KD Observed bind size: 44KD
Product group Antibodies
Boster Bio
Anti-MEK4/MAP2K4 Antibody Picoband(r)PA2275-DYLIGHT594
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetMAP2K4
  • SizePrice
Anti-MEK4 antibody, PA2275, All Western blotting All lanes: Anti-MAP2K4(PA2275) at 0.5ug/ml Lane 1: Rat Skeletal Muscle Tissue Lysate at 40ug Lane 2: HELA Whole Cell Lysate at 40ug Lane 3: A549 Whole Cell Lysate at 40ug Lane 4: MM231 Whole Cell Lysate at 40ug Lane 5: CEM Whole Cell Lysate at 40ug Predicted bind size: 44KD Observed bind size: 44KD
Product group Antibodies
Boster Bio
Anti-MEK4/MAP2K4 Antibody Picoband(r)PA2275-FITC
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetMAP2K4
  • SizePrice
Anti-MEK4 antibody, PA2275, All Western blotting All lanes: Anti-MAP2K4(PA2275) at 0.5ug/ml Lane 1: Rat Skeletal Muscle Tissue Lysate at 40ug Lane 2: HELA Whole Cell Lysate at 40ug Lane 3: A549 Whole Cell Lysate at 40ug Lane 4: MM231 Whole Cell Lysate at 40ug Lane 5: CEM Whole Cell Lysate at 40ug Predicted bind size: 44KD Observed bind size: 44KD
Product group Antibodies
Boster Bio
Anti-MEK4/MAP2K4 Antibody Picoband(r)PA2275-HRP
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetMAP2K4
  • SizePrice
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