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Search results: MAPK14 p38 P38 MAPK

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Product group Antibodies
References
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetMAPK14
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Lane 1: NIH/3T3 lysates; Lane 2: Human brain lysates; Line 3: 293 lysates ;Probed with p38 MAPK (10B7)Monoclonal Antibody (bsm-52844R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Product group Antibodies
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetMAPK14
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Product group Antibodies
Assay Genie
ReactivityHuman
TargetMAPK14
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Product group Antibodies
Assay Genie
ReactivityHuman
TargetMAPK14
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Tissue/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (phospho-p38 MAPK (Thr180 + Tyr182)) polyclonal Antibody, Unconjugated (bs-2210R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Product group Antibodies
References
Bioss Antibodies
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityCanine, Chicken, Equine, Goat, Human, Mouse, Porcine, Rabbit, Rat
TargetMAPK14
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Product group Antibodies
Biorbyt
ApplicationsImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetMAPK14
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Product group Antibodies
Biorbyt
ApplicationsImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetMAPK14
  • SizePrice
Western blot analysis of p38 MAPK expression in Jurkat cell lysate (M00176-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAPK14 monoclonal antibody (Catalog # M00176-1) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
Product group Antibodies
References
Boster Bio
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetMAPK14
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
ApplicationsWestern Blot
ReactivityHuman, Mouse
TargetMAPK14
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Product group Antibodies
LifeSpan BioSciences
ApplicationsWestern Blot
ReactivityHuman
TargetMAPK14
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
ApplicationsWestern Blot
ReactivityHuman, Mouse
TargetMAPK14
  • SizePrice
Product group Antibodies
Biorbyt
ApplicationsELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityCanine, Chicken, Equine, Human, Mouse, Porcine, Rabbit, Rat
TargetMAPK14
  • SizePrice