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Search results: MAPK14 p38 P38 MAPK

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Western blot analysis of CrkII phosphorylation expression in COS7 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.
Product group Antibodies
OriGene
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetCRK
  • SizePrice
Western blot analysis of extracts of various cell lines, using CRK/CRKII antibody (TA375097) at 1:1000 dilution. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% nonfat dry milk in TBST.
Product group Antibodies
OriGene
ApplicationsWestern Blot
ReactivityHuman, Mouse
TargetCRK
  • SizePrice
Western blot analysis of extracts of K562 cells, using Phospho-CRK/CRKII-Y221 antibody (TA375098) at 1:1000 dilution. K562 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37°C for 1 hour . - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% BSA. - Detection: ECL Basic Kit . - Exposure time: 10s.
Product group Antibodies
OriGene
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetCRK
  • SizePrice
WB Suggested Anti-CRK Antibody Titration: 1.0 ug/ml Positive Control: Transfected 293T
Product group Antibodies
OriGene
ApplicationsWestern Blot
ReactivityHuman
TargetCRK
  • SizePrice
Immunohistochemistry of paraffin-embedded Human colon cancer tissue using TA349831 (CRK Antibody) at dilution 1/50 (Original magnification: x200)
Product group Antibodies
OriGene
ApplicationsImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetCRK
  • SizePrice
Immunohistochemistry of paraffin-embedded Human cervical cancer tissue using TA364733 (CRK Antibody) at dilution 1/40 (Original magnification: x200)
Product group Antibodies
OriGene
ApplicationsImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetCRK
  • SizePrice
Host: Rabbit Target Name: CRK Sample Tissue: Human Jurkat Whole Cell lysates Antibody Dilution: 1ug/ml
Product group Antibodies
OriGene
ApplicationsWestern Blot
ReactivityHuman
TargetCRK
  • SizePrice
Western blot analysis of AIMP2 using anti-AIMP2 antibody (PA1481). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat lung tissue lysates, Lane 3: rat kidney tissue lysates, Lane 4: rat brain tissue lysates, Lane 5: Jurkat cell lysates, Lane 6: CEM cell lysates, Lane 7: HUT cell lysates, Lane 8: U93T cell lysates, Lane 9: U93T cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AIMP2 antigen affinity purified polyclonal antibody (Catalog # PA1481) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetAIMP2
  • SizePrice
Product group Antibodies
Biorbyt
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetMAPK1
  • SizePrice
Product group Antibodies
References
Biorbyt
ApplicationsImmunoFluorescence, Western Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetMAPK1
  • SizePrice
Product group Antibodies
Biorbyt
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetMAPK1
  • SizePrice
Product group Antibodies
Biorbyt
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetMAPK12
  • SizePrice