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Search results: MBD1

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Immunofluorescent staining of human cell line HaCaT shows localization to nucleoplasm & vesicles.
Product group Antibodies
Atlas Antibodies
Anti-MBD1HPA068850
ApplicationsWestern Blot, ImmunoCytoChemistry
ReactivityHuman
TargetMBD1
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Product group Antibodies
BPS Bioscience
ApplicationsWestern Blot, ChIP Chromatin ImmunoPrecipitation, ELISA
ReactivityHuman
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GTX110612 WB Image
Product group Antibodies
References
GeneTex
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Western blot analysis of MBD1 expression in HeLa cell lysate using anti-MBD1 antibody (M02336). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MBD1 antigen affinity purified polyclonal antibody (Catalog # M02336) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
Product group Antibodies
Boster Bio
Product group Antibodies
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityBovine, Canine, Equine, Guinea Pig, Human, Mammals, Monkey, Mouse, Porcine, Rabbit, Rat
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Product group Antibodies
LifeSpan BioSciences
ApplicationsWestern Blot
ReactivityHuman, Monkey
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Product group Antibodies
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityBovine, Canine, Equine, Guinea Pig, Human, Mammals, Monkey, Porcine, Rabbit
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Product group Proteins / Signaling Molecules
LifeSpan BioSciences
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Product group Proteins / Signaling Molecules
LifeSpan BioSciences
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Product group Proteins / Signaling Molecules
LifeSpan BioSciences
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Product group Proteins / Signaling Molecules
LifeSpan BioSciences
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GTX60359 ChIP assay Image
Product group Antibodies
GeneTex
ApplicationsWestern Blot, ChIP Chromatin ImmunoPrecipitation, ELISA
ReactivityHuman, Mouse
  • SizePrice