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Search results: MNK1

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Product group Proteins / Signaling Molecules
OriGene
Protein IDQ63844
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Figure 1. Western blot analysis of MAP kinase,activated using anti-MAP kinase,activated antibody (MA1055). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-MAP kinase,activated antigen affinity purified monoclonal antibody (Catalog # MA1055) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for MAP kinase,activated at approximately 42 kDa. The expected band size for MAP kinase,activated is at 41 kDa.
Product group Antibodies
References
Boster Bio
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityBovine, Human, Monkey, Mouse, Rat, Yeast
TargetMAPK1
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Product group Chemicals
AbMole BioScience
CAS Number522629-08-9
Estimated Purity>98%
Molecular Weight244.23
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Product group Chemicals
MedChem Express
ETC-168HY-156511
Molecular Weight409.44
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CgoaEGY7SNqEMYL0AAAAAE3bT0Q287.png
Product group Chemicals
TargetMol Chemicals
CAS Number522629-08-9
Estimated Purity99.89%
Molecular Weight244.23
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Product group Chemicals
TargetMol Chemicals
Molecular Weight409.44
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Product group Chemicals
Selleck Chemicals
CAS Number1849590-01-7
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Product group Chemicals
MedChem Express
EB1HY-146805
Estimated Purity99.91
Molecular Weight286.33
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CgoaEWY7T-iEB69IAAAAAGSLzkE754.png
Product group Chemicals
TargetMol Chemicals
Estimated Purity99.82%
Molecular Weight286.33
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Product group Antibodies
Aviva Systems Biology
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityMouse
TargetMapk3
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CgoaEWY7SzyEV2HxAAAAABIxxtQ488.png
Product group Chemicals
TargetMol Chemicals
CAS Number848942-61-0
Estimated Purity98.35%
Molecular Weight473.93
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Product group Antibodies
ABclonal
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetMKNK1
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