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Figure 1. Western blot analysis of PDCD4 using anti-PDCD4 antibody (A01105). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Daudi whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDCD4 antigen affinity purified polyclonal antibody (Catalog # A01105) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDCD4 at approximately 60 kDa. The expected band size for PDCD4 is at 60 kDa.
Product group Antibodies
Boster Bio
Anti-PDCD4 Antibody Picoband(r)A01105-IFLUOR647
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
ReactivityHuman
TargetPDCD4
  • SizePrice
Figure 1. Western blot analysis of PDCD4 using anti-PDCD4 antibody (A01105). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Daudi whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDCD4 antigen affinity purified polyclonal antibody (Catalog # A01105) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDCD4 at approximately 60 kDa. The expected band size for PDCD4 is at 60 kDa.
Product group Antibodies
Boster Bio
Anti-PDCD4 Antibody Picoband(r)A01105-PE
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
ReactivityHuman
TargetPDCD4
  • SizePrice
Figure 1. Western blot analysis of PDCD4 using anti-PDCD4 antibody (A01105). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Daudi whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDCD4 antigen affinity purified polyclonal antibody (Catalog # A01105) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDCD4 at approximately 60 kDa. The expected band size for PDCD4 is at 60 kDa.
Product group Antibodies
Boster Bio
Anti-PDCD4 Antibody Picoband(r)A01105
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
ReactivityHuman
TargetPDCD4
  • SizePrice
Product group Antibodies
Abbexa
PDCD4 (pS457) AntibodyABX333383
ApplicationsWestern Blot, ELISA
  • SizePrice
Product group Antibodies
Abbexa
PDCD4 (pS67) AntibodyABX333395
ApplicationsWestern Blot, ELISA
  • SizePrice
Product group Proteins / Signaling Molecules
Atlas Antibodies
PrEST Antigen PDCD4APREST76140
Protein IDQ53EL6
  • SizePrice
Product group Proteins / Signaling Molecules
Atlas Antibodies
PrEST Antigen PDCD4APREST76141
Protein IDQ53EL6
  • SizePrice
Human PDCD4 ELISA Kit
Product group Assays
Antibodies.com
Human PDCD4 ELISA KitA77101
Assay Sample TypePlasma, tissue homogenates and other biological fluids.
ReactivityHuman
  • SizePrice
Western blot analysis of extracts from HepG2 tissue using Phospho-PDCD4 (Ser67) antibody.The lane on the right is treated with the antigen-specific peptide.
Product group Antibodies
Cusabio
Phospho-PDCD4 (Ser67) AntibodyCSB-PA047784
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetPDCD4
  • SizePrice
Product group Antibodies
MedChem Express
PDCD4 Antibody (YA240, Rabbit)HY-P80269
TargetPDCD4
  • SizePrice
3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Product group Proteins / Signaling Molecules
Boster Bio
Human PDCD4 Recombinant ProteinPROTQ53EL6
ApplicationsOther Application
Protein IDQ53EL6
  • SizePrice
3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Product group Proteins / Signaling Molecules
Boster Bio
Human PDCD4 Recombinant ProteinPROTQ53EL6
ApplicationsOther Application
Protein IDQ53EL6
  • SizePrice
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