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Figure 1. Western blot analysis of PDCD4 using anti-PDCD4 antibody (A01105-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat RH35 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDCD4 antigen affinity purified polyclonal antibody (Catalog # A01105-4) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDCD4 at approximately 64 kDa. The expected band size for PDCD4 is at 52 kDa.
Product group Antibodies
Boster Bio
Anti-PDCD4 Antibody Picoband(r)A01105-4-DYLIGHT594
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetPDCD4
  • SizePrice
Figure 1. Western blot analysis of PDCD4 using anti-PDCD4 antibody (A01105-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat RH35 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDCD4 antigen affinity purified polyclonal antibody (Catalog # A01105-4) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDCD4 at approximately 64 kDa. The expected band size for PDCD4 is at 52 kDa.
Product group Antibodies
Boster Bio
Anti-PDCD4 Antibody Picoband(r)A01105-4-FITC
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetPDCD4
  • SizePrice
Figure 1. Western blot analysis of PDCD4 using anti-PDCD4 antibody (A01105-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat RH35 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDCD4 antigen affinity purified polyclonal antibody (Catalog # A01105-4) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDCD4 at approximately 64 kDa. The expected band size for PDCD4 is at 52 kDa.
Product group Antibodies
Boster Bio
Anti-PDCD4 Antibody Picoband(r)A01105-4-HRP
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetPDCD4
  • SizePrice
Figure 1. Western blot analysis of PDCD4 using anti-PDCD4 antibody (A01105-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat RH35 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDCD4 antigen affinity purified polyclonal antibody (Catalog # A01105-4) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDCD4 at approximately 64 kDa. The expected band size for PDCD4 is at 52 kDa.
Product group Antibodies
Boster Bio
Anti-PDCD4 Antibody Picoband(r)A01105-4-IFLUOR647
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetPDCD4
  • SizePrice
Figure 1. Western blot analysis of PDCD4 using anti-PDCD4 antibody (A01105-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat RH35 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDCD4 antigen affinity purified polyclonal antibody (Catalog # A01105-4) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDCD4 at approximately 64 kDa. The expected band size for PDCD4 is at 52 kDa.
Product group Antibodies
Boster Bio
Anti-PDCD4 Antibody Picoband(r)A01105-4-PE
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetPDCD4
  • SizePrice
Figure 1. Western blot analysis of PDCD4 using anti-PDCD4 antibody (A01105-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat RH35 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDCD4 antigen affinity purified polyclonal antibody (Catalog # A01105-4) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDCD4 at approximately 64 kDa. The expected band size for PDCD4 is at 52 kDa.
Product group Antibodies
Boster Bio
Anti-PDCD4 Antibody Picoband(r)A01105-4
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetPDCD4
  • SizePrice
Product group Assays
MyBioSource
PDCD4 Western Blot kitMBS3224718
  • SizePrice
WB analysis of normal (control) and knockout (KO) HeLa cell lysate using GTX35232 PDCD4 antibody. Dilution : 1:1000 Loading : 25microg per lane
Product group Antibodies
GeneTex
PDCD4 antibodyGTX35232
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse
TargetPDCD4
  • SizePrice
Product group Assays
Biomatik
Pdcd4 ELISA KitEKA50464
Assay Sample TypeResearchers plate their cell line of choice
ReactivityHuman, Mouse, Rat
  • SizePrice
Anti-PDCD4 (Phospho-Ser457) Antibody
Product group Antibodies
Antibodies.com
Anti-PDCD4 (Phospho-Ser457) AntibodyA50991
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
  • SizePrice
Anti-PDCD4 (phospho Ser67) Antibody
Product group Antibodies
Antibodies.com
Anti-PDCD4 (phospho Ser67) AntibodyA94028
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
  • SizePrice
Anti-PDCD4 (phospho Ser457) Antibody
Product group Antibodies
Antibodies.com
Anti-PDCD4 (phospho Ser457) AntibodyA94178
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
  • SizePrice
7891011

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