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Search results: Phos-tag Agarose

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Product group Proteins / Signaling Molecules
MyBioSource
Triticum vulgare, Succinylated (WGA, Wheat Germ, Agglutinin) (Agarose)MBS656216
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Product group Technique Specific Products
Santa Cruz Biotechnology
ChIP-Grade Protein A/G Plus Agarosesc-500775
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Product group Technique Specific Products
Santa Cruz Biotechnology
ChIP-Grade Protein A/G Plus AgaroseSC-500775A
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Product group Antibodies
MyBioSource
HA agaroseMBS395087
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Product group Antibodies
OriGene
Mouse Monoclonal tGFP Antibody, Clone OTI2H8, Agarose BeadsTA183081
ApplicationsImmunoPrecipitation, Purification/Extraction/Isolation
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Product group Antibodies
OriGene
Mouse Monoclonal tGFP Antibody, Clone OTI2H8, Agarose BeadsTA183081S
ApplicationsImmunoPrecipitation, Purification/Extraction/Isolation
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Immunoprecipitation was performed with HEK293T cell lysates of expressing Entry vector (control, Cat# PS100001) or expressing DDK-tagged PON3 (Cat# LY400339). Anti-DDK 4C5-agarose beads were incubated with control lysate or LY400339 overnight at 4°C. After washing three times with lysate buffer, 20uL 4x SDS sample buffer was applied. The samples were boiled at 95°C for 10min. The supernatant was loaded to the SDS-PAGE gel after discarded the beads by centrifuging. After transferring to the membrane, the Western blot assay was applied with HRP-conjugated anti-DDK antibody (Cat# TA150030). Lane 1: control lysate; Lane 2: DDK-tagged PON3 (LY400339); Lane 3: anti-DDK 4C5 agarose beads (TA150037) 50uL with control lysate; Lane 4: anti-DDK 4C5 agarose beads (TA150037) 50uL with LY400339.
Product group Antibodies
OriGene
Clone OTI4C5, Anti-DDK (FLAG) Monoclonal Antibody, Agarose BeadsTA150037
ApplicationsImmunoPrecipitation, Purification/Extraction/Isolation
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Immunoprecipitation was performed with HEK293T cell lysates of expressing Entry vector (control, Cat# PS100001) or expressing DDK-tagged PON3 (Cat# LY400339). Anti-DDK 4C5-agarose beads were incubated with control lysate or LY400339 overnight at 4°C. After washing three times with lysate buffer, 20uL 4x SDS sample buffer was applied. The samples were boiled at 95°C for 10min. The supernatant was loaded to the SDS-PAGE gel after discarded the beads by centrifuging. After transferring to the membrane, the Western blot assay was applied with HRP-conjugated anti-DDK antibody (Cat# TA150030). Lane 1: control lysate; Lane 2: DDK-tagged PON3 (LY400339); Lane 3: anti-DDK 4C5 agarose beads (TA150037) 50uL with control lysate; Lane 4: anti-DDK 4C5 agarose beads (TA150037) 50uL with LY400339.
Product group Antibodies
OriGene
Clone OTI4C5, Anti-DDK (FLAG) Monoclonal Antibody, Agarose BeadsTA150037S
ApplicationsImmunoPrecipitation, Purification/Extraction/Isolation
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Product group Antibodies
MyBioSource
Agarose Conjugated Anti-HA Tag Mouse Monoclonal Antibody (4F6)MBS9719135
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Product group Antibodies
MyBioSource
Agarose Conjugated Anti-His Tag Mouse Monoclonal Antibody (5C3)MBS9719140
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Product group Antibodies
MyBioSource
Agarose Conjugated Anti-Myc Tag Mouse Monoclonal Antibody (2D5)MBS9719145
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Product group Antibodies
MyBioSource
Agarose Conjugated Anti-V5 Tag Mouse Monoclonal Antibody (11D5)MBS9719158
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