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Search results: Siglec-3

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Western blot analysis of CTCF expression in (1) HeLa cell lysate; (2) Mouse brain lysate (M00132). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CTCF monoclonal antibody (Catalog # M00132) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CTCF
Product group Antibodies
Boster Bio
Anti-CTCF Rabbit Monoclonal AntibodyM00132
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetCTCF
  • SizePrice
Western blot analysis of CD14 expression in Human tonsil cell lysate (M00137). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD14 monoclonal antibody (Catalog # M00137) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD14
Product group Antibodies
Boster Bio
Anti-CD14 Rabbit Monoclonal AntibodyM00137
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetCD14
  • SizePrice
Western blot analysis of CD19 expression in (1) Jurkat cell lysate; (2) Rat spleen lysate.
Product group Antibodies
Boster Bio
Anti-CD19 Rabbit Monoclonal AntibodyM00154-1
ApplicationsFlow Cytometry, Western Blot
ReactivityHuman, Mouse, Rat
TargetCD19
  • SizePrice
Western blot analysis of CD19 expression in Ramos cell lysate.
Product group Antibodies
Boster Bio
Anti-CD19 Rabbit Monoclonal AntibodyM00154
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetCD19
  • SizePrice
Immunohistochemical analysis of paraffin-embedded human tonsil, using CCR2/CKR2.
Product group Antibodies
Boster Bio
Anti-CCR2/CKR2 Rabbit Monoclonal AntibodyM00158
ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetCCR2
  • SizePrice
Western blot analysis of CDK4 expression in HeLa cell lysate (M00159-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDK4 monoclonal antibody (Catalog # M00159-1) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDK4
Product group Antibodies
Boster Bio
Anti-CDK4 Rabbit Monoclonal AntibodyM00159-1
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetCDK4
  • SizePrice
Western blot analysis of CDK4 expression in HeLa cell lysate (M00159). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDK4 monoclonal antibody (Catalog # M00159) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDK4
Product group Antibodies
Boster Bio
Anti-CDK4 Rabbit Monoclonal AntibodyM00159
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetCDK4
  • SizePrice
Western blot analysis of C3 expression in Human plasma lysate using anti-C3 antibody (M00168-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-C3 antigen affinity purified polyclonal antibody (Catalog # M00168-2) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for C3.
Product group Antibodies
Boster Bio
Anti-Complement C3 Monoclonal AntibodyM00168-2
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman
TargetC3
  • SizePrice
Western blot analysis of C3 expression in HepG2 cell lysate.
Product group Antibodies
Boster Bio
Anti-C3/Complement C3 Rabbit Monoclonal AntibodyM00168
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman
TargetC3
  • SizePrice
Western blot analysis of CDK9 expression in HeLa cell lysate (M00794). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDK9 monoclonal antibody (Catalog # M00794) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDK9
Product group Antibodies
Boster Bio
Anti-CDK9 Rabbit Monoclonal AntibodyM00794
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetCDK9
  • SizePrice
Immunohistochemical analysis of paraffin-embedded human colon, using CDX2 Antibody.
Product group Antibodies
Boster Bio
Anti-CDX2 Rabbit Monoclonal AntibodyM00877-1
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetCDX2
  • SizePrice
Western blot analysis of Calreticulin expression in (1)HeLa cell lysate; (2)HepG2 cell lysate.
Product group Antibodies
Boster Bio
Anti-Calreticulin Rabbit Monoclonal AntibodyM00894
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetCALR
  • SizePrice
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