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Search results: Siglec-3

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Western blot analysis of CD59 expression in HUVEC cell lysate (M00914-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD59 monoclonal antibody (Catalog # M00914-1) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD59
Product group Antibodies
Boster Bio
Anti-CD59 Rabbit Monoclonal AntibodyM00914-1
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
ReactivityHuman
TargetCD59
  • SizePrice
Western blot analysis of Cystatin C expression in HeLa cell lysate (M00961). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CST3 monoclonal antibody (Catalog # M00961) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CST3
Product group Antibodies
Boster Bio
Anti-Cystatin C CST3 Rabbit Monoclonal AntibodyM00961
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetCST3
  • SizePrice
Western blot analysis of Caveolin 3 expression in Human fetal heart lysate (M00990-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CAV3 monoclonal antibody (Catalog # M00990-1) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CAV3
Product group Antibodies
Boster Bio
Anti-Caveolin-3 CAV3 Rabbit Monoclonal AntibodyM00990-1
ApplicationsImmunoPrecipitation, Western Blot
ReactivityHuman, Mouse, Rat
TargetCAV3
  • SizePrice
Western blot analysis of Caveolin 3 expression in Human fetal heart lysate (M00990). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CAV3 monoclonal antibody (Catalog # M00990) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CAV3
Product group Antibodies
Boster Bio
Anti-Caveolin-3 CAV3 Rabbit Monoclonal AntibodyM00990
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetCAV3
  • SizePrice
Immunohistochemical analysis of paraffin-embedded human kidney, using C9 Antibody.
Product group Antibodies
Boster Bio
Anti-C9/Complement Component C9 Rabbit Monoclonal AntibodyM01010
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
ReactivityHuman
TargetC9
  • SizePrice
Western blot analysis of CNPase expression in C6 cell lysate (M01017). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CNP monoclonal antibody (Catalog # M01017) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CNP
Product group Antibodies
Boster Bio
Anti-CNPase Rabbit Monoclonal AntibodyM01017
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetCNP
  • SizePrice
Western blot analysis of CDT1 expression in HEK293 cell lysate.
Product group Antibodies
Boster Bio
Anti-CDT1 Rabbit Monoclonal AntibodyM01035
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetCDT1
  • SizePrice
Western blot analysis of Caspase-7 expression in NIH/3T3 cell lysate (M01044-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Casp7 monoclonal antibody (Catalog # M01044-1) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Casp7
Product group Antibodies
Boster Bio
Anti-Caspase-7 Casp7 Rabbit Monoclonal AntibodyM01044-1
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
ReactivityMouse, Rat
TargetCasp7
  • SizePrice
Western blot analysis of CD79A using anti-CD79A antibody (M01047-1) in human spleen lysate. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD79A antigen affinity purified polyclonal antibody (Catalog # M01047-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
Product group Antibodies
Boster Bio
Anti-CD79a Monoclonal AntibodyM01047-1
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetCD79A
  • SizePrice
Western blot analysis of Chk1 in (1) K562 cell lysate; (2) PC-12 cell lysate (M01060-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CHEK1 monoclonal antibody (Catalog # M01060-1) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CHEK1
Product group Antibodies
Boster Bio
Anti-Chk1 CHEK1 Rabbit Monoclonal AntibodyM01060-1
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetCHEK1
  • SizePrice
Western blot analysis of Chk1 expression in HeLa cell lysate.
Product group Antibodies
Boster Bio
Anti-Chk1 CHEK1 Rabbit Monoclonal AntibodyM01060
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetCHEK1
  • SizePrice
Immunohistochemical analysis of paraffin-embedded human spleen, using CD63 Antibody.
Product group Antibodies
Boster Bio
Anti-CD63 Rabbit Monoclonal AntibodyM01080-1
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman
TargetCD63
  • SizePrice
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