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Search results: Siglec-3

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Western blot analysis of CPT2 expression in MCF-7 cell lysate (M02112). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CPT2 monoclonal antibody (Catalog # M02112) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CPT2
Product group Antibodies
Boster Bio
Anti-CPT2 Rabbit Monoclonal AntibodyM02112
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetCPT2
  • SizePrice
Western blot analysis of Cdc37 expression in (1) Jurkat cell lysate; (2) SW480 cell lysate.
Product group Antibodies
Boster Bio
Anti-Cdc37 Rabbit Monoclonal AntibodyM02169
ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetCDC37
  • SizePrice
Western blot analysis of Caspase-10 expression in Jurkat cell lysate.
Product group Antibodies
Boster Bio
Anti-Caspase-10 Rabbit Monoclonal AntibodyM02190-1
ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
ReactivityHuman
TargetCASP10
  • SizePrice
Western blot analysis of CRX using anti-CRX antibody on Y79 cell lysate(M02202). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CRX antigen affinity purified polyclonal antibody (Catalog # M02202) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
Product group Antibodies
Boster Bio
Anti-CRX Monoclonal AntibodyM02202
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman
TargetCRX
  • SizePrice
Western blot analysis of CD276 expression in 293T cell lysate (M02220). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD276 monoclonal antibody (Catalog # M02220) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD276
Product group Antibodies
Boster Bio
Anti-CD276/B7 H3 Rabbit Monoclonal AntibodyM02220
ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot
ReactivityHuman, Mouse
TargetCD276
  • SizePrice
Western blot analysis of Collagen VI expression in Human brain lysate.
Product group Antibodies
Boster Bio
Anti-Collagen VI COL6A1 Rabbit Monoclonal AntibodyM02226
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetCOL6A1
  • SizePrice
Immunohistochemical analysis of paraffin-embedded human colon, using CD8 Antibody(M02236-1) CD8A was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CD8A Antibody (M02236-1)overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
Boster Bio
Anti-CD8 CD8A Rabbit Monoclonal AntibodyM02236-1
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Rat
TargetCD8A
  • SizePrice
Immunohistochemical analysis of paraffin-embedded human spleen, using CD8 alpha Antibody.
Product group Antibodies
Boster Bio
Anti-CD8 alpha Rabbit Monoclonal AntibodyM02236-2
ApplicationsImmunoHistoChemistry
ReactivityHuman
TargetCD8A
  • SizePrice
Western blot analysis of CD82 expression in Jurkat cell lysate using anti-CD82 antibody (M02300). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD82 antigen affinity purified polyclonal antibody (Catalog # M02300) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for CD82.
Product group Antibodies
Boster Bio
Anti-CD82 Monoclonal AntibodyM02300
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Rat
TargetCD82
  • SizePrice
Western blot analysis of delta1 Catenin expression in HeLa cell lysate (M02333). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CTNND1 monoclonal antibody (Catalog # M02333) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CTNND1
Product group Antibodies
Boster Bio
Anti-delta 1 Catenin/p120 Catenin Rabbit Monoclonal AntibodyM02333
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetCTNND1
  • SizePrice
Western blot analysis of CCR9 in (1) A549 cell lysate; (2) Daudi cell lysate.
Product group Antibodies
Boster Bio
Anti-CCR9 Rabbit Monoclonal AntibodyM02348
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetCCR9
  • SizePrice
Immunohistochemical analysis of paraffin-embedded mouse testis, using Caspase-2 Antibody(M02384-1) CASP2 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CASP2 Antibody (M02384-1)overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
Boster Bio
Anti-Caspase-2 CASP2 Rabbit Monoclonal AntibodyM02384-1
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetCASP2
  • SizePrice
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