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Search results: Siglec-3

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Western blot analysis of CD74 expression in Ramos cell lysate (M01340-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD74 monoclonal antibody (Catalog # M01340-2) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD74
Product group Antibodies
Boster Bio
Anti-CD74 Rabbit Monoclonal AntibodyM01340-2
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetCD74
  • SizePrice
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
Product group Antibodies
Boster Bio
Anti-Cdc25C Rabbit Monoclonal AntibodyM01343-30UL
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetCDC25C
  • SizePrice
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
Product group Antibodies
Boster Bio
Anti-Cdc25C Rabbit Monoclonal AntibodyM01343
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetCDC25C
  • SizePrice
Western Blot
Product group Antibodies
Boster Bio
Anti-Human VEGI Monoclonal Antibody Biotin Conjugated, Flow ValidatedFC02402-BIOTIN
ApplicationsWestern Blot, ImmunoCytoChemistry
ReactivityHuman
TargetTNFSF15
  • SizePrice
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
Product group Antibodies
Boster Bio
Anti-CCR3 Rabbit Monoclonal AntibodyM01748-30UL
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetCCR3
  • SizePrice
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
Product group Antibodies
Boster Bio
Anti-CCR3 Rabbit Monoclonal AntibodyM01748
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetCCR3
  • SizePrice
Western blot analysis of CDC40 expression in (1) HeLa cell lysate; (2) K562 cell lysate.
Product group Antibodies
Boster Bio
Anti-CDC40 Rabbit Monoclonal AntibodyM07786-30UL
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetCDC40
  • SizePrice
Western blot analysis of CDC40 expression in (1) HeLa cell lysate; (2) K562 cell lysate.
Product group Antibodies
Boster Bio
Anti-CDC40 Rabbit Monoclonal AntibodyM07786
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetCDC40
  • SizePrice
All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
Product group Antibodies
Boster Bio
Anti-Calponin CNN1 Rabbit Monoclonal AntibodyM08065-1-30UL
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetCNN1
  • SizePrice
All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
Product group Antibodies
References
Boster Bio
Anti-Calponin CNN1 Rabbit Monoclonal AntibodyM08065-1
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetCNN1
  • SizePrice
Figure 1. Western blot analysis of CDCA3 using anti-CDCA3 antibody (M10074). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: rat heart tissue lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse heart tissue lysates, Lane 7: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDCA3 antigen affinity purified monoclonal antibody (Catalog # M10074) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDCA3 at approximately 29 kDa. The expected band size for CDCA3 is at 29 kDa.
Product group Antibodies
Boster Bio
Anti-CDCA3 Rabbit Monoclonal AntibodyM10074-30UL
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetCDCA3
  • SizePrice
Figure 1. Western blot analysis of CDCA3 using anti-CDCA3 antibody (M10074). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: rat heart tissue lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse heart tissue lysates, Lane 7: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDCA3 antigen affinity purified monoclonal antibody (Catalog # M10074) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDCA3 at approximately 29 kDa. The expected band size for CDCA3 is at 29 kDa.
Product group Antibodies
Boster Bio
Anti-CDCA3 Rabbit Monoclonal AntibodyM10074
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetCDCA3
  • SizePrice
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