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Search results: USP14

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Product group Proteins / Signaling Molecules
SignalChem
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Product group Proteins / Signaling Molecules
SignalChem
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Product group Proteins / Signaling Molecules
MyBioSource
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Product group Antibodies
Reddot Biotech
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse
TargetUSP14
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USP14 activity is inhibited by Ub-Aldehyde.
Product group Assays
BPS Bioscience
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Western blot analysis of USP14 expression in (1) HeLa cell lysate; (2) RAW 2647 cell lysate; (3) C6 cell lysate (M03042). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-USP14 monoclonal antibody (Catalog # M03042) overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for USP14
Product group Antibodies
Boster Bio
Product group Proteins / Signaling Molecules
LifeSpan BioSciences
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Product group Assays
LifeSpan BioSciences
ReactivityHuman
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Product group Assays
LifeSpan BioSciences
ReactivityHuman
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Product group Antibodies
LifeSpan BioSciences
ApplicationsWestern Blot
ReactivityHuman
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More sizes
Product group Molecular Biology
Sino Biological
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Product group Molecular Biology
Sino Biological
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