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Search results: Ubiquitin AMC

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Figure 1. Western blot analysis of UBA1 using anti-UBA1 antibody (PB9904). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse liver tissue lysates. Lane 3: mouse testis tissue lysates. Lane 4: HELA whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UBA1 antigen affinity purified polyclonal antibody (Catalog # PB9904) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UBA1 at approximately 117 kDa. The expected band size for UBA1 is at 118 kDa.
Product group Antibodies
Boster Bio
Anti-E1 Ubiquitin Activating Enzyme/UBA1 Antibody Picoband(r)PB9904-HRP
ApplicationsWestern Blot
ReactivityBovine, Equine, Human, Monkey, Mouse, Rabbit, Rat
TargetUBA1
  • SizePrice
Figure 1. Western blot analysis of UBA1 using anti-UBA1 antibody (PB9904). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse liver tissue lysates. Lane 3: mouse testis tissue lysates. Lane 4: HELA whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UBA1 antigen affinity purified polyclonal antibody (Catalog # PB9904) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UBA1 at approximately 117 kDa. The expected band size for UBA1 is at 118 kDa.
Product group Antibodies
Boster Bio
Anti-E1 Ubiquitin Activating Enzyme/UBA1 Antibody Picoband(r)PB9904-IFLUOR647
ApplicationsWestern Blot
ReactivityBovine, Equine, Human, Monkey, Mouse, Rabbit, Rat
TargetUBA1
  • SizePrice
Figure 1. Western blot analysis of UBA1 using anti-UBA1 antibody (PB9904). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse liver tissue lysates. Lane 3: mouse testis tissue lysates. Lane 4: HELA whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UBA1 antigen affinity purified polyclonal antibody (Catalog # PB9904) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UBA1 at approximately 117 kDa. The expected band size for UBA1 is at 118 kDa.
Product group Antibodies
Boster Bio
Anti-E1 Ubiquitin Activating Enzyme/UBA1 Antibody Picoband(r)PB9904-PE
ApplicationsWestern Blot
ReactivityBovine, Equine, Human, Monkey, Mouse, Rabbit, Rat
TargetUBA1
  • SizePrice
Figure 1. Western blot analysis of UBA1 using anti-UBA1 antibody (PB9904). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse liver tissue lysates. Lane 3: mouse testis tissue lysates. Lane 4: HELA whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UBA1 antigen affinity purified polyclonal antibody (Catalog # PB9904) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UBA1 at approximately 117 kDa. The expected band size for UBA1 is at 118 kDa.
Product group Antibodies
Boster Bio
Anti-E1 Ubiquitin Activating Enzyme/UBA1 Antibody Picoband(r)PB9904
ApplicationsWestern Blot
ReactivityBovine, Equine, Human, Monkey, Mouse, Rabbit, Rat
TargetUBA1
  • SizePrice
Product group Molecular Biology
List Labs
MAPKKide Plus (AMC) Specific Substrate for Anthrax Lethal Factor532
  • SizePrice
Product group Chemicals
Bachem
Pyr-AMC4004069
CAS Number66642-36-2
Molecular Weight286.29
  • SizePrice
Product group Proteins / Signaling Molecules
ABclonal
Ub-AMCRP10247DLQ
Protein IDP0CG47
  • SizePrice
Product group Chemicals
MyBioSource
Arachidonoyl-AMCMBS565550
Estimated Purity>=99%
  • SizePrice
Product group Proteins / Signaling Molecules
MyBioSource
Met-AMCMBS566470
  • SizePrice
Product group Proteins / Signaling Molecules
MyBioSource
TRH-AMCMBS8247106
  • SizePrice
Product group Proteins / Signaling Molecules
MyBioSource
Z-D-E-V-D-AMCMBS8247152
  • SizePrice
Product group Proteins / Signaling Molecules
MyBioSource
Z-F-R-AMCMBS826344
  • SizePrice
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