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Figure 1. Western blot analysis of WAC using anti-WAC antibody (A04941-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human Raji whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human U20S whole cell lysates, Lane 6: human SK-OV-3 whole cell lysates, Lane 7: rat brain tissue lysates, Lane 8: rat PC-12 whole cell lysates, Lane 9: mouse thymus tissue lysates, Lane 10: mouse SP2/0 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WAC antigen affinity purified polyclonal antibody (Catalog # A04941-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WAC at approximately 85KD. The expected band size for WAC is at 85KD.
Product group Antibodies
Boster Bio
Anti-WAC Antibody Picoband(r)A04941-1-IFLUOR647
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
TargetWAC
  • SizePrice
Figure 1. Western blot analysis of WAC using anti-WAC antibody (A04941-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human Raji whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human U20S whole cell lysates, Lane 6: human SK-OV-3 whole cell lysates, Lane 7: rat brain tissue lysates, Lane 8: rat PC-12 whole cell lysates, Lane 9: mouse thymus tissue lysates, Lane 10: mouse SP2/0 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WAC antigen affinity purified polyclonal antibody (Catalog # A04941-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WAC at approximately 85KD. The expected band size for WAC is at 85KD.
Product group Antibodies
Boster Bio
Anti-WAC Antibody Picoband(r)A04941-1-PE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
TargetWAC
  • SizePrice
Figure 1. Western blot analysis of WAC using anti-WAC antibody (A04941-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human Raji whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human U20S whole cell lysates, Lane 6: human SK-OV-3 whole cell lysates, Lane 7: rat brain tissue lysates, Lane 8: rat PC-12 whole cell lysates, Lane 9: mouse thymus tissue lysates, Lane 10: mouse SP2/0 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WAC antigen affinity purified polyclonal antibody (Catalog # A04941-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WAC at approximately 85KD. The expected band size for WAC is at 85KD.
Product group Antibodies
Boster Bio
Anti-WAC Antibody Picoband(r)A04941-1
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
TargetWAC
  • SizePrice
Product group Antibodies
Cusabio
wac AntibodyCSB-PA319157XA01EDZ
ApplicationsWestern Blot, ELISA
ReactivityVirus
Targetwac
  • SizePrice
Western Blot Positive WB detected in Recombinant protein All lanes: wac antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 67.7 kDa Observed band size: 68 kDa
Product group Antibodies
Cusabio
wac AntibodyCSB-PA319157ZA01EDZ
ApplicationsWestern Blot, ELISA
ReactivityVirus
Targetwac
  • SizePrice
Product group Antibodies
Bioss
WAC Polyclonal Antibody, APC ConjugatedBS-12787R-APC
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
TargetWAC
  • SizePrice
Product group Antibodies
Bioss
WAC Polyclonal Antibody, Biotin ConjugatedBS-12787R-BIOTIN
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetWAC
  • SizePrice
Product group Antibodies
Bioss
WAC Polyclonal Antibody, Cy3 ConjugatedBS-12787R-CY3
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
TargetWAC
  • SizePrice
Product group Antibodies
Bioss
WAC Polyclonal Antibody, Cy5.5 ConjugatedBS-12787R-CY5.5
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
TargetWAC
  • SizePrice
Product group Antibodies
Bioss
WAC Polyclonal Antibody, Cy5 ConjugatedBS-12787R-CY5
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
TargetWAC
  • SizePrice
Product group Antibodies
Bioss
WAC Polyclonal Antibody, Cy7 ConjugatedBS-12787R-CY7
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
TargetWAC
  • SizePrice
Product group Antibodies
Bioss
WAC Polyclonal Antibody, FITC ConjugatedBS-12787R-FITC
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
TargetWAC
  • SizePrice
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