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Figure 1. Western blot analysis of WAC using anti-WAC antibody (A04941-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human Raji whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human U20S whole cell lysates, Lane 6: human SK-OV-3 whole cell lysates, Lane 7: rat brain tissue lysates, Lane 8: rat PC-12 whole cell lysates, Lane 9: mouse thymus tissue lysates, Lane 10: mouse SP2/0 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WAC antigen affinity purified polyclonal antibody (Catalog # A04941-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WAC at approximately 85KD. The expected band size for WAC is at 85KD.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
TargetWAC
  • SizePrice
Figure 1. Western blot analysis of WAC using anti-WAC antibody (A04941-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human Raji whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human U20S whole cell lysates, Lane 6: human SK-OV-3 whole cell lysates, Lane 7: rat brain tissue lysates, Lane 8: rat PC-12 whole cell lysates, Lane 9: mouse thymus tissue lysates, Lane 10: mouse SP2/0 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WAC antigen affinity purified polyclonal antibody (Catalog # A04941-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WAC at approximately 85KD. The expected band size for WAC is at 85KD.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
TargetWAC
  • SizePrice
Figure 1. Western blot analysis of WAC using anti-WAC antibody (A04941-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human Raji whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human U20S whole cell lysates, Lane 6: human SK-OV-3 whole cell lysates, Lane 7: rat brain tissue lysates, Lane 8: rat PC-12 whole cell lysates, Lane 9: mouse thymus tissue lysates, Lane 10: mouse SP2/0 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WAC antigen affinity purified polyclonal antibody (Catalog # A04941-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WAC at approximately 85KD. The expected band size for WAC is at 85KD.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
TargetWAC
  • SizePrice
Product group Antibodies
Cusabio
wac AntibodyCSB-PA319157XA01EDZ
ApplicationsWestern Blot, ELISA
ReactivityVirus
Targetwac
  • SizePrice
Western Blot Positive WB detected in Recombinant protein All lanes: wac antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 67.7 kDa Observed band size: 68 kDa
Product group Antibodies
Cusabio
wac AntibodyCSB-PA319157ZA01EDZ
ApplicationsWestern Blot, ELISA
ReactivityVirus
Targetwac
  • SizePrice
Product group Antibodies
Bioss
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
TargetWAC
  • SizePrice
Product group Antibodies
Bioss
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetWAC
  • SizePrice
Product group Antibodies
Bioss
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
TargetWAC
  • SizePrice
Product group Antibodies
Bioss
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
TargetWAC
  • SizePrice
Product group Antibodies
Bioss
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
TargetWAC
  • SizePrice
Product group Antibodies
Bioss
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
TargetWAC
  • SizePrice
Product group Antibodies
Bioss
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
TargetWAC
  • SizePrice

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