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Search results: WAC

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Product group Molecular Biology
Sino Biological
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Anti-WAC Antibody
Product group Antibodies
Antibodies.com
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
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Western blot analysis of WAC in human testis tissue lysate with WAC antibody at 1 microg/mL.
Product group Antibodies
Boster Bio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetWAC
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Product group Antibodies
Biomatik
ApplicationsWestern Blot, ELISA
ReactivityVirus
Targetwac
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Product group Antibodies
Bioss
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityBovine, Canine, Chicken, Equine, Human, Mouse, Porcine, Rabbit, Rat, Sheep
TargetWAC
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Immunohistochemical staining of human hippocampus shows strong nuclear positivity in neuronal cells.
Product group Antibodies
Atlas Antibodies
ApplicationsImmunoHistoChemistry
ReactivityHuman
TargetWAC
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Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
Product group Antibodies
Atlas Antibodies
ApplicationsImmunoCytoChemistry
ReactivityHuman
TargetWAC
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Product group Antibodies
Cusabio
ApplicationsELISA
ReactivityHuman
TargetWAC
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Product group Antibodies
Cusabio
ReactivityHuman
TargetWAC
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Product group Antibodies
Cusabio
ApplicationsELISA
ReactivityHuman
TargetWAC
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Figure 1. Western blot analysis of WAC using anti-WAC antibody (A04941-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human Raji whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human U20S whole cell lysates, Lane 6: human SK-OV-3 whole cell lysates, Lane 7: rat brain tissue lysates, Lane 8: rat PC-12 whole cell lysates, Lane 9: mouse thymus tissue lysates, Lane 10: mouse SP2/0 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WAC antigen affinity purified polyclonal antibody (Catalog # A04941-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WAC at approximately 85KD. The expected band size for WAC is at 85KD.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetWAC
  • SizePrice
Figure 1. Western blot analysis of WAC using anti-WAC antibody (A04941-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human Raji whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human U20S whole cell lysates, Lane 6: human SK-OV-3 whole cell lysates, Lane 7: rat brain tissue lysates, Lane 8: rat PC-12 whole cell lysates, Lane 9: mouse thymus tissue lysates, Lane 10: mouse SP2/0 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WAC antigen affinity purified polyclonal antibody (Catalog # A04941-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WAC at approximately 85KD. The expected band size for WAC is at 85KD.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetWAC
  • SizePrice

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