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Western blot analysis of extracts of MCF7 cells, using AKT1 antibody (TA373412) at 1:1000 dilution. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% nonfat dry milk in TBST. - Detection: ECL Basic Kit . - Exposure time: 60s.
Product group Antibodies
OriGene
AKT1 Rabbit Polyclonal AntibodyTA373412
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
Western blot analysis of extracts of various cell lines, using Phospho-AKT1-S473 pAb (TA373415) at 1:1000 dilution.NIH/3T3 cells were treated by Calyculin A (100 nM) at 37°C for 30 minutes after serum-starvation overnight. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% nonfat dry milk in TBST. - Detection: ECL Basic Kit . - Exposure time: 10s.
Product group Antibodies
OriGene
AKT1 Rabbit Polyclonal AntibodyTA373415
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
Western blot analysis of extracts of HeLa cells, using Phospho-AKT1-T308 antibody (TA373416) at 1:1000 dilution.HeLa cells were treated by PDGF (100 ng/ml) at 37°C for 30 minutes after serum-starvation overnight. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% nonfat dry milk in TBST. - Detection: ECL Basic Kit . - Exposure time: 180s.
Product group Antibodies
OriGene
AKT1 Rabbit Polyclonal AntibodyTA373416
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
Western blot analysis of extracts of 293T cells, using Phospho-AKT1-S129 antibody (TA373417) at 1:500 dilution.293T cells were treated by CIP(20uL/400ul) at 37°C for 1 hour. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% nonfat dry milk in TBST. - Detection: ECL Enhanced Kit . - Exposure time: 180s.
Product group Antibodies
OriGene
AKT1 Rabbit Polyclonal AntibodyTA373417
ApplicationsWestern Blot
ReactivityHuman
TargetAKT1
  • SizePrice
Western blot analysis of extracts of various cell lines, using Phospho-AKT1-S473+AKT2-S474+AKT3-S472 antibody (TA373419) at 1:1000 dilution.NIH/3T3 cells were treated by Calyculin A (100 nM) at 37°C for 30 minutes after serum-starvation overnight.C6 cells were treated by 10% FBS at 37°C for 30 minutes after serum-starvation overnight. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% nonfat dry milk in TBST. - Detection: ECL Basic Kit . - Exposure time: 10s.
Product group Antibodies
OriGene
AKT1 Rabbit Polyclonal AntibodyTA373419
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
Western Blot of Mouse Anti-AKT1 Antibody. Lane 1: Opal Prestained Molecular Weight Protein (p/n MB-210-0500). Lane 2: AKT1 protein (p/n 009-001-P21). Lane 3: AKT2 protein (p/n 009-001-P22). Lane 4: AKT3 protein (p/n 009-001-P23). Load: 50ng. Blocking: BlockOut Buffer (p/n MB-073) for 30 min at RT. Primary Antibody: Anti-AKT1 at 1ug/mL o/n at 4°C. Secondary Antibody: Rabbit Anti-Mouse IgG HRP (p/n 610-403-C46, Lot 20121) at 1:40,000 in MB-073 for 30 min at RT.
Product group Antibodies
OriGene
AKT1 Mouse Monoclonal Antibody [Clone ID: 14E5.A2.B2.H9]TA396813
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetAKT1
  • SizePrice
Western Blot of Mouse Anti-AKT1 Antibody. Lane 1: Opal Prestained Molecular Weight Protein (p/n MB-210-0500). Lane 2: AKT1 protein (p/n 009-001-P21). Lane 3: AKT2 protein (p/n 009-001-P22). Lane 4: AKT3 protein (p/n 009-001-P23). Load: 50ng. Blocking: BlockOut Buffer (p/n MB-073) for 30 min at RT. Primary Antibody: Anti-AKT1 at 1ug/mL o/n at 4°C. Secondary Antibody: Rabbit Anti-Mouse IgG HRP (p/n 610-403-C46, Lot 20121) at 1:40,000 in MB-073 for 30 min at RT.
Product group Antibodies
OriGene
AKT1 Mouse Monoclonal Antibody [Clone ID: 14E5.A2.B2.H9]TA396814S
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetAKT1
  • SizePrice
Western Blot of Mouse anti-AKT1 antibody. Lane 1: GST-AKT1. Lane 2: GST-AKT2. Lane 3: GST-AKT3. Lane 4: Molecular Weight Marker. Load: 25 ng per lane. Primary antibody: AKT1 antibody at 1:1000 for overnight at 4°C. Secondary antibody: Mouse secondary antibody at 1:40,000 for 30 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 78 kDa for AKT1. Other band(s): none.
Product group Antibodies
OriGene
AKT1 Mouse Monoclonal Antibody [Clone ID: 5E5.F5.D7]TA396815
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetAKT1
  • SizePrice
Western Blot of Mouse anti-AKT1 antibody. Lane 1: GST-AKT1. Lane 2: GST-AKT2. Lane 3: GST-AKT3. Lane 4: Molecular Weight Marker. Load: 25 ng per lane. Primary antibody: AKT1 antibody at 1:1000 for overnight at 4°C. Secondary antibody: Mouse secondary antibody at 1:40,000 for 30 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 78 kDa for AKT1. Other band(s): none.
Product group Antibodies
OriGene
AKT1 Mouse Monoclonal Antibody [Clone ID: 5E5.F5.D7]TA396815S
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetAKT1
  • SizePrice
Western Blot of Mouse anti-AKT1 antibody. Lane 1: GST-AKT1. Lane 2: GST-AKT2. Lane 3: GST-AKT3. Lane 4: Molecular Weight Marker. Load: 25 ng per lane. Primary antibody: AKT1 antibody at 1:1000 for overnight at 4°C. Secondary antibody: Mouse secondary antibody at 1:40,000 for 30 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 78 kDa for AKT1. Other band(s): none.
Product group Antibodies
OriGene
AKT1 Mouse Monoclonal Antibody [Clone ID: 5E5.F5.D7]TA396816S
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetAKT1
  • SizePrice
Western Blot of Mouse anti-AKT1 antibody. Lane 1: GST-AKT1. Lane 2: GST-AKT2. Lane 3: GST-AKT3. Lane 4: Molecular Weight Marker. Load: 25 ng per lane. Primary antibody: AKT1 antibody at 1:1000 for overnight at 4°C. Secondary antibody: Mouse secondary antibody at 1:40,000 for 30 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 78 kDa for AKT1. Other band(s): none.
Product group Antibodies
OriGene
AKT1 Mouse Monoclonal Antibody [Clone ID: 5E5.F5.D7]TA396828
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
Western Blot of Mouse anti-AKT1 antibody. Lane 1: GST Tagged recombinant AKT1. Lane 2: GST Tagged recombinant AKT2. Lane 3: GST Tagged recombinant AKT3. Load: 25 ng per lane. Primary antibody: AKT1 unconjugated antibody at 1:1,000 for overnight at 4°C. Secondary antibody: Peroxidase Rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 78 kDa for AKT2. Other band(s): none.
Product group Antibodies
OriGene
AKT1 Mouse Monoclonal Antibody [Clone ID: 14E5.A2.B2.H9]TA396841
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
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