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HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY AKT3 (Cat# RC221051, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKT3 (Cat# TA500001).
Product group Antibodies
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetAKT3
  • SizePrice
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKT3 (RC221051, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKT3. Positive lysates LY401675 (100ug) and LC401675 (20ug) can be purchased separately from OriGene.
Product group Antibodies
OriGene
ApplicationsImmunoFluorescence, Western Blot
ReactivityHuman, Mouse, Rat
TargetAKT3
  • SizePrice
Immunofluorescence of Mouse monoclonal anti-AKT3 antibody. Cell Type: A431 cells. Fixation: 4% paraformaldehyde 10 min. Permeablization: 0.5% Triton X 30 min. Primary Ab: (p/n 200-301-J36) at 1:250 for 72 hours 4°C. Secondary Ab: (p/n 610-142-121) at 1:1000 overnight 4°C.
Product group Antibodies
OriGene
ApplicationsWestern Blot
ReactivityHuman
TargetAKT3
  • SizePrice
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY AKT3 (Cat# RC221051, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKT3 (Cat# TA500001).
Product group Antibodies
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetAKT3
  • SizePrice
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY AKT3 (Cat# RC221051, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKT3 (Cat# TA500001).
Product group Antibodies
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetAKT3
  • SizePrice
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY AKT3 (Cat# RC221051, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKT3 (Cat# TA500001).
Product group Antibodies
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetAKT3
  • SizePrice
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY AKT3 (Cat# RC221051, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKT3 (Cat# TA500001).
Product group Antibodies
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetAKT3
  • SizePrice
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKT3 (RC221051, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKT3. Positive lysates LY401675 (100ug) and LC401675 (20ug) can be purchased separately from OriGene.
Product group Antibodies
OriGene
ApplicationsImmunoFluorescence, Western Blot
ReactivityHuman, Mouse, Rat
TargetAKT3
  • SizePrice
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKT3 (RC221051, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKT3. Positive lysates LY401675 (100ug) and LC401675 (20ug) can be purchased separately from OriGene.
Product group Antibodies
OriGene
ApplicationsImmunoFluorescence, Western Blot
ReactivityHuman, Mouse, Rat
TargetAKT3
  • SizePrice
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKT3 (RC221051, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKT3. Positive lysates LY401675 (100ug) and LC401675 (20ug) can be purchased separately from OriGene.
Product group Antibodies
OriGene
ApplicationsImmunoFluorescence, Western Blot
ReactivityHuman, Mouse, Rat
TargetAKT3
  • SizePrice
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKT3 (RC221051, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKT3. Positive lysates LY401675 (100ug) and LC401675 (20ug) can be purchased separately from OriGene.
Product group Antibodies
OriGene
ApplicationsImmunoFluorescence, Western Blot
ReactivityHuman, Mouse, Rat
TargetAKT3
  • SizePrice
Western blot analysis of extracts of various cell lines, using AKT3 antibody (TA373426) at 1:3000 dilution. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% nonfat dry milk in TBST. - Detection: ECL Basic Kit . - Exposure time: 90s.
Product group Antibodies
OriGene
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetAKT3
  • SizePrice