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Western Blot of Mouse anti-Akt phospho T308 Biotin Conjugated antibody. Lane 1: GST tagged AKT1 active recombinant protein. Lane 2: GST tagged AKT1 un-active recombinant protein. Load: 25 ng per lane. Primary antibody: Akt phospho T308 Biotin Conjugated antibody at 1:1,000 for overnight at 4°C. Secondary antibody: HRP Streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 79 kDa, 79 kDa for Akt phospho T308. Other band (s): none
Product group Antibodies
OriGene
AKT1 Mouse Monoclonal Antibody [Clone ID: 18F3.H11]TA319561
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Monkey, Mouse, Rat
TargetAKT1
  • SizePrice
WB of Mouse Anti-AKT pS473 antibody. Lane 1: non-phosphorylated AKT in untreated cells. Lane 2: phosphorylated AKT (indicated by arrowhead at ~56 kDa) on PDGF stimulated NIH/3T3 cell lysates. Load: 10 ug per lane. Primary antibody: AKT pS473 antibody at 1:10,000 in TBS with 0.05% Tween-20 with 1% BSA, for 1 h at 4°C. Secondary antibody: HRP conjugated Gt-a-Mouse IgG (p/n 610-103-121) was used at a 1:20,000 dilution.
Product group Antibodies
OriGene
AKT1 Mouse Monoclonal Antibody [Clone ID: 17F6.B11]TA319562
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Monkey, Mouse, Rat
TargetAKT1
  • SizePrice
Western Blot of Mouse anti-Akt phospho S473 Biotin Conjugated antibody. Lane 1: GST tagged AKT1 active recombinant protein. Lane 2: none. Load: 25 ng per lane. Primary antibody: Akt phospho S473 Biotin Conjugated antibody at 1:1,000 for overnight at 4°C. Secondary antibody: HRP Streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 79 kDa, 79 kDa for Akt phospho S473. Other band (s): none
Product group Antibodies
OriGene
AKT1 Mouse Monoclonal Antibody [Clone ID: 17F6.B11]TA319563
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Monkey, Mouse, Rat
TargetAKT1
  • SizePrice
Western blot analysis of Akt expression in Serum treated NIH-3T3 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.
Product group Antibodies
OriGene
AKT1 Rabbit Polyclonal AntibodyTA325213
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
Western blot analysis of Akt expression in Serum treated HuvEc whole cell lysates, The lane on the left is treated with the antigen-specific peptide.
Product group Antibodies
OriGene
AKT1 Rabbit Polyclonal AntibodyTA325214
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
Western blot analysis on 293 cell lysate using Akt Antibody
Product group Antibodies
OriGene
AKT1 Rabbit Polyclonal AntibodyTA325215
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
Western blot analysis of Akt phosphorylation expression in Mouse braintissue lysates, The lane on the left is treated with the antigen-specific peptide.
Product group Antibodies
OriGene
AKT1 Rabbit Polyclonal AntibodyTA325216
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
Western blot analysis of Akt phosphorylation expression in PMA treated A549 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.
Product group Antibodies
OriGene
AKT1 Rabbit Polyclonal AntibodyTA325217
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
Western blot analysis of Akt phosphorylation expression in heatshock treated HeLa whole cell lysates, The lane on the left is treated with the antigen-specific peptide.
Product group Antibodies
OriGene
AKT1 Rabbit Polyclonal AntibodyTA325218
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
Western blot analysis of Akt phosphorylation expression in Insulin treated 293 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.
Product group Antibodies
OriGene
AKT1 Rabbit Polyclonal AntibodyTA325219
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
Western blot analysis on 293 cell lysate using Phospho-Akt (Ser473) Antibody
Product group Antibodies
OriGene
AKT1 Rabbit Polyclonal AntibodyTA325221
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
Western blot analysis of NIH3T3 cells starved overnight (lane 1), NIH3T3 cells starved overnight and treated with PDGF 100ng/ml for 15min (lane 2) and Jurkat (lane 3) cells using anti-AKT phospho (ser473)antibody (poly6490). beta-actin antibody (poly6221) was used as loading control.
Product group Antibodies
OriGene
AKT1 Rabbit Polyclonal Antibody [Clone ID: Poly6490]TA328006
ApplicationsWestern Blot
ReactivityHuman, Mouse
TargetAKT1
  • SizePrice
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